PAX 8 activates the enhancer of the human thyroperoxidase gene

Claudio Esposito, Stefania Miccadei, Adolfe Saiardi, Donato Civitareale

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Abstract

In this study we report on a novel natural target of the paired domain transcription factor PAX 8 in the enhancer element of the human thyroperoxidase gene, one of the most important thyroid differentiation marker. It is the primary enzymen involved in the thyroid hormone synthesis an PAX 8 has been previously identified as as activating factor of the rat thyroperoxidase gene promoter. In vitro, PAX 8 binds a cis element of the human enhancer and its exogenous expression induces the enhancer activity in co-transfection experiments in Cos-7 cells. When mutated at this binding site, the enhancer is no longer activated by PAX 8. Our finding strengthens the PAX 8 role in the maintenance of thyroid differentiation and in particular in the tissue-specific thyroperoxidase gene expression.

Original languageEnglish
Pages (from-to)37-40
Number of pages4
JournalBiochemical Journal
Volume331
Issue number1
Publication statusPublished - Apr 1 1998

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ASJC Scopus subject areas

  • Biochemistry

Cite this

Esposito, C., Miccadei, S., Saiardi, A., & Civitareale, D. (1998). PAX 8 activates the enhancer of the human thyroperoxidase gene. Biochemical Journal, 331(1), 37-40.