PC-1 content in skeletal muscle of non-obese, non-diabetic subjects: Relationship to insulin receptor tyrosine kinase and whole body insulin sensitivity

L. Frittitta, J. Youngren, R. Vigneri, B. A. Maddux, V. Trischitta, I. D. Goldfine

Research output: Contribution to journalArticlepeer-review

Abstract

Insulin sensitivity varies widely in non-obese, non-diabetic subjects, and we have previously reported that in vivo insulin action correlates with in vitro insulin stimulated insulin receptor tyrosine-kinase activity in skeletal muscle. Plasma membrane glycoprotein PC-1 content is elevated in fibroblasts of insulin-resistant subjects, and expression of PC-1 cDNA in cultured cells reduces both insulin receptor tyrosine-kinase activity and the biological actions of insulin. In the present study we investigated non-obese, non-diabetic subjects and found a significant negative correlation between muscle PC-1 content and both in vivo insulin action as measured by the intravenous insulin tolerance test (r = -0.51, p = 0.035) and the sensitivity (ED50) of in vitro insulin stimulation of insulin receptor tyrosine-kinase activity (r = 0.66, p = 0.027). These studies indicate, therefore, that increased muscle PC-1 content is associated with reduced insulin action both in vivo and in vitro. Moreover, they suggest possible role for PC-1 in regulating insulin receptor function in human skeletal muscle.

Original languageEnglish
Pages (from-to)1190-1195
Number of pages6
JournalDiabetologia
Volume39
Issue number10
Publication statusPublished - 1996

Keywords

  • Insulin receptor tyrosine-kinase activity
  • Insulin sensitivity
  • Insulin signalling
  • Insulin tolerance test
  • Skeletal muscle

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Internal Medicine

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