PCR analysis of HIV-1 sequences and differential immunological features in seronegative and seropositive haemophiliacs

C. Chelucci, H. J. Hassan, A. Gringeri, G. Macioce, G. Mariani, E. Santagostino, U. Testa, F. Vulcano, P. M. Mannucci, C. Peschle

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Abstract

We have compared the immunological features of two matched groups of seronegative and seropositive haemophilia A individuals. Both groups were exposed from 1981 to 1985 to comparable amounts and batches of FVIII concentrates not subjected to virus inactivation procedures, and had therefore a 100% probability of receiving HIV-contaminated material. The presence of proviral HIV-1 sequences was evaluated by PCR in the DNA from peripheral blood lymphocytes and/or monocytes. After hybridization with specific probes, DNA from all seropositive haemophiliacs revealed HIV sequences; no HIV sequences were observed from the DNA of seronegative patients, even after two rounds of amplification, thus suggesting that these patients were not affected by a latent HIV infection. Seronegative/PCR - and seropositive/PCR + patients showed a normal and reduced number of CD4 + lymphocytes, and a slight and marked increase of CD8 + cells respectively. Activated T cells expressing the HLA-DR antigen were elevated in both groups. Interestingly, a significant reduction of circulating CD56 +/CD3 - NK lymphocytes was observed only in seropositive haemophiliacs, whereas NK lymphocytes with CD56 +/CD3 + phenotype were within normal levels in both groups. In seropositive patients no correlation was found between the number of CD4 + and CD56 +/CD3 - lymphocytes. The marked reduction of CD56 +/CD3 - lymphocytes observed in seropositive haemophiliacs in addition to the CD4 + cell depletion may represent a key pathogenetic factor which facilitates the onset and/or the progression of HIV-1 infection in haemophiliacs, and is related to the capacity of HIV to infect NK cells.

Original languageEnglish
Pages (from-to)558-567
Number of pages10
JournalBritish Journal of Haematology
Volume81
Issue number4
Publication statusPublished - 1992

ASJC Scopus subject areas

  • Hematology

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    Chelucci, C., Hassan, H. J., Gringeri, A., Macioce, G., Mariani, G., Santagostino, E., Testa, U., Vulcano, F., Mannucci, P. M., & Peschle, C. (1992). PCR analysis of HIV-1 sequences and differential immunological features in seronegative and seropositive haemophiliacs. British Journal of Haematology, 81(4), 558-567.