PCR assay to detect Bacillus anthracis spores in heat-treated specimens

A. Fasanella, S. Losito, R. Adone, F. Ciuchini, T. Trotta, S. A. Altamura, D. Chiocco, G. Ippolito

Research output: Contribution to journalArticlepeer-review

Abstract

Recent interest in anthrax is due to its potential use in bioterrorism and as a biowarfare agent against civilian populations. The development of rapid and sensitive techniques to detect anthrax spores in suspicious specimens is the most important aim for public health. With a view to preventing exposure of laboratory workers to viable Bacillus anthracis spores, this study evaluated the suitability of PCR assays for detecting anthrax spores previously inactivated at 121°C for 45 min. The results indicate that heat treatment ensures the complete inactivation of B. anthracis spores without significantly affecting the efficiency of PCR assays.

Original languageEnglish
Pages (from-to)896-899
Number of pages4
JournalJournal of Clinical Microbiology
Volume41
Issue number2
DOIs
Publication statusPublished - Feb 1 2003

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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