PCR-based immortalization and screening of hierarchical pools of cDNAs

Maurizio D'Esposito, Richard Mazzarella, Gina Pengue, Carmela Jones, Michele D'Urso, David Schelessinger

Research output: Contribution to journalArticle

Abstract

Starting from sequences of at least 60 bp, PCR-based screening has been developed to recover cDNAs from libraries without the necessity for hybridization or extensive DNA extraction steps. The method maintains the indefinite availability of even scarce cDNA libraries and provides an estimate of the relative abundance of the mRNA species. Isolation of a cDNA clone can be done in less than a week. cDNAs were isolated that were cognate for fragments of expressed sequences and for an exon predicted from genomic sequence.

Original languageEnglish
Pages (from-to)4806-4809
Number of pages4
JournalNucleic Acids Research
Volume22
Issue number22
Publication statusPublished - Nov 11 1994

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

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    D'Esposito, M., Mazzarella, R., Pengue, G., Jones, C., D'Urso, M., & Schelessinger, D. (1994). PCR-based immortalization and screening of hierarchical pools of cDNAs. Nucleic Acids Research, 22(22), 4806-4809.