PCR identification of Helicobacter pylori DNA in faeces from patients with gastroduodenal pathology

Maria Notarnicola, Francesco Russo, Aldo Cavallini, Maria Bianco, Emilio Jirillo, Salvatore Pece, Claudio Leoci, Giovanni Di Matteo, Alfredo Di Leo

Research output: Contribution to journalArticle

Abstract

Polymerase chain reaction (PCR) may represent a sensitive method for Helicobacter pylori (Hp) detection in faeces, even if different chemicals, such as polysaccharides, can give false negative results by inhibiting the amplification reaction. Our aim was to investigate by PCR the prevalence of Hp in the faeces of patients infected with this bacterium, removing the faecal PCR inhibitors by an appropriate filtration step. DNA was extracted from faeces of 61 patients undergoing endoscopy and subsequently amplified. FOR products were separated and identified by electrophoresis. PCR assay detected Hp DNA in 44 of 46 (95.6%) patients who resulted in being histologically positive. No Hp DNA was found in Hp-negative patients. These findings may suggest a faecal-oral route of Hp transmission, even if Hp DNA may be also due to DNA from non-viable, or viable but non-infectious, cells present in faeces.

Original languageEnglish
Pages (from-to)785-787
Number of pages3
JournalMedical Science Research
Volume24
Issue number11
Publication statusPublished - 1996

Keywords

  • Faeces
  • Helicobacter pylori
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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    Notarnicola, M., Russo, F., Cavallini, A., Bianco, M., Jirillo, E., Pece, S., Leoci, C., Matteo, G. D., & Leo, A. D. (1996). PCR identification of Helicobacter pylori DNA in faeces from patients with gastroduodenal pathology. Medical Science Research, 24(11), 785-787.