TY - JOUR
T1 - PCR-RFLP analysis of the Cryptosporidium oocyst wall protein (COWP) gene discriminates between C. wrairi and C. parvum, and between C. parvum isolates of human and animal origin
AU - Spano, Furio
AU - Putignani, Lorenza
AU - McLauchlin, James
AU - Casemore, David P.
AU - Crisanti, Andrea
PY - 1997/5/15
Y1 - 1997/5/15
N2 - Cryptosporidium wrairi was isolated from guinea pigs during a spontaneous outbreak of cryptosporidiosis. Despite the morphological and antigenic similarities to C. parvum, C. wrairi displayed a different host range and site of infection and may represent a separate species or sub- species. We used the polymerase chain reaction to clone two distinct 550 bp- long DNA fragments, Wc-I and Wc-II, of the gene encoding the Cryptosporidium oocyst wall protein (COWP) of C. wrairi, which showed 98% identity to the C. parvum homologue. Within Wc-I, polymorphic RsaI restriction sites were used to develop a polymerase chain reaction-restriction fragment length polymorphism method able to distinguish C. wrairi from C. parvum and to identify two groups of C. parvum isolates differentially associated with animal and human infections.
AB - Cryptosporidium wrairi was isolated from guinea pigs during a spontaneous outbreak of cryptosporidiosis. Despite the morphological and antigenic similarities to C. parvum, C. wrairi displayed a different host range and site of infection and may represent a separate species or sub- species. We used the polymerase chain reaction to clone two distinct 550 bp- long DNA fragments, Wc-I and Wc-II, of the gene encoding the Cryptosporidium oocyst wall protein (COWP) of C. wrairi, which showed 98% identity to the C. parvum homologue. Within Wc-I, polymorphic RsaI restriction sites were used to develop a polymerase chain reaction-restriction fragment length polymorphism method able to distinguish C. wrairi from C. parvum and to identify two groups of C. parvum isolates differentially associated with animal and human infections.
KW - Cryptosporidium oocyst wall protein gene
KW - Cryptosporidium parvum
KW - Cryptosporidium wrairi
KW - Genetic marker
KW - Polymerase chain reaction
UR - http://www.scopus.com/inward/record.url?scp=0030902945&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030902945&partnerID=8YFLogxK
U2 - 10.1016/S0378-1097(97)00115-8
DO - 10.1016/S0378-1097(97)00115-8
M3 - Article
C2 - 9170264
AN - SCOPUS:0030902945
VL - 150
SP - 209
EP - 217
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
SN - 0378-1097
IS - 2
ER -