PD-1 in human NK cells: evidence of cytoplasmic mRNA and protein expression

Francesca R Mariotti, Stefania Petrini, Tiziano Ingegnere, Nicola Tumino, Francesca Besi, Francesca Scordamaglia, Enrico Munari, Silvia Pesce, Emanuela Marcenaro, Alessandro Moretta, Paola Vacca, Lorenzo Moretta

Research output: Contribution to journalArticle

Abstract

Under physiological conditions, PD-1/PD-L1 interactions regulate unwanted over-reactions of immune cells and contribute to maintain peripheral tolerance. However, in tumor microenvironment, this interaction may greatly compromise the immune-mediated anti-tumor activity. PD-1+ NK cells have been detected in high percentage in peripheral blood and ascitic fluid of ovarian carcinoma patients. To acquire information on PD-1 expression and physiology in human NK cells, we analyzed whether PD-1 mRNA and protein are present in resting, surface PD-1-, NK cells from healthy donors. Both different splicing isoforms of PD-1 mRNA and a cytoplasmic pool of PD-1 protein were detected. Similar results were obtained also from both in vitro-activated and tumor-associated NK cells. PD-1 mRNA and protein were higher in CD56dim than in CD56bright NK cells. Confocal microscopy analyses revealed that PD-1 protein is present in virtually all NK cells analyzed. The present findings are compatible with a rapid surface expression of PD-1 in NK cells in response to appropriate, still undefined, stimuli.

Original languageEnglish
Pages (from-to)1557030
JournalOncoImmunology
Volume8
Issue number3
DOIs
Publication statusPublished - Dec 25 2019

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Natural Killer Cells
Messenger RNA
Proteins
Peripheral Tolerance
Tumor Microenvironment
Ascitic Fluid
Confocal Microscopy
Neoplasms
Protein Isoforms
Tissue Donors
Carcinoma

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PD-1 in human NK cells : evidence of cytoplasmic mRNA and protein expression. / Mariotti, Francesca R; Petrini, Stefania; Ingegnere, Tiziano; Tumino, Nicola; Besi, Francesca; Scordamaglia, Francesca; Munari, Enrico; Pesce, Silvia; Marcenaro, Emanuela; Moretta, Alessandro; Vacca, Paola; Moretta, Lorenzo.

In: OncoImmunology, Vol. 8, No. 3, 25.12.2019, p. 1557030.

Research output: Contribution to journalArticle

Mariotti, Francesca R ; Petrini, Stefania ; Ingegnere, Tiziano ; Tumino, Nicola ; Besi, Francesca ; Scordamaglia, Francesca ; Munari, Enrico ; Pesce, Silvia ; Marcenaro, Emanuela ; Moretta, Alessandro ; Vacca, Paola ; Moretta, Lorenzo. / PD-1 in human NK cells : evidence of cytoplasmic mRNA and protein expression. In: OncoImmunology. 2019 ; Vol. 8, No. 3. pp. 1557030.
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