Abstract
Under physiological conditions, PD-1/PD-L1 interactions regulate unwanted over-reactions of immune cells and contribute to maintain peripheral tolerance. However, in tumor microenvironment, this interaction may greatly compromise the immune-mediated anti-tumor activity. PD-1+ NK cells have been detected in high percentage in peripheral blood and ascitic fluid of ovarian carcinoma patients. To acquire information on PD-1 expression and physiology in human NK cells, we analyzed whether PD-1 mRNA and protein are present in resting, surface PD-1-, NK cells from healthy donors. Both different splicing isoforms of PD-1 mRNA and a cytoplasmic pool of PD-1 protein were detected. Similar results were obtained also from both in vitro-activated and tumor-associated NK cells. PD-1 mRNA and protein were higher in CD56dim than in CD56bright NK cells. Confocal microscopy analyses revealed that PD-1 protein is present in virtually all NK cells analyzed. The present findings are compatible with a rapid surface expression of PD-1 in NK cells in response to appropriate, still undefined, stimuli.
| Original language | English |
|---|---|
| Pages (from-to) | 1557030 |
| Journal | OncoImmunology |
| Volume | 8 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Dec 25 2019 |
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PD-1 in human NK cells : evidence of cytoplasmic mRNA and protein expression. / Mariotti, Francesca R; Petrini, Stefania; Ingegnere, Tiziano; Tumino, Nicola; Besi, Francesca; Scordamaglia, Francesca; Munari, Enrico; Pesce, Silvia; Marcenaro, Emanuela; Moretta, Alessandro; Vacca, Paola; Moretta, Lorenzo.
In: OncoImmunology, Vol. 8, No. 3, 25.12.2019, p. 1557030.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - PD-1 in human NK cells
T2 - evidence of cytoplasmic mRNA and protein expression
AU - Mariotti, Francesca R
AU - Petrini, Stefania
AU - Ingegnere, Tiziano
AU - Tumino, Nicola
AU - Besi, Francesca
AU - Scordamaglia, Francesca
AU - Munari, Enrico
AU - Pesce, Silvia
AU - Marcenaro, Emanuela
AU - Moretta, Alessandro
AU - Vacca, Paola
AU - Moretta, Lorenzo
PY - 2019/12/25
Y1 - 2019/12/25
N2 - Under physiological conditions, PD-1/PD-L1 interactions regulate unwanted over-reactions of immune cells and contribute to maintain peripheral tolerance. However, in tumor microenvironment, this interaction may greatly compromise the immune-mediated anti-tumor activity. PD-1+ NK cells have been detected in high percentage in peripheral blood and ascitic fluid of ovarian carcinoma patients. To acquire information on PD-1 expression and physiology in human NK cells, we analyzed whether PD-1 mRNA and protein are present in resting, surface PD-1-, NK cells from healthy donors. Both different splicing isoforms of PD-1 mRNA and a cytoplasmic pool of PD-1 protein were detected. Similar results were obtained also from both in vitro-activated and tumor-associated NK cells. PD-1 mRNA and protein were higher in CD56dim than in CD56bright NK cells. Confocal microscopy analyses revealed that PD-1 protein is present in virtually all NK cells analyzed. The present findings are compatible with a rapid surface expression of PD-1 in NK cells in response to appropriate, still undefined, stimuli.
AB - Under physiological conditions, PD-1/PD-L1 interactions regulate unwanted over-reactions of immune cells and contribute to maintain peripheral tolerance. However, in tumor microenvironment, this interaction may greatly compromise the immune-mediated anti-tumor activity. PD-1+ NK cells have been detected in high percentage in peripheral blood and ascitic fluid of ovarian carcinoma patients. To acquire information on PD-1 expression and physiology in human NK cells, we analyzed whether PD-1 mRNA and protein are present in resting, surface PD-1-, NK cells from healthy donors. Both different splicing isoforms of PD-1 mRNA and a cytoplasmic pool of PD-1 protein were detected. Similar results were obtained also from both in vitro-activated and tumor-associated NK cells. PD-1 mRNA and protein were higher in CD56dim than in CD56bright NK cells. Confocal microscopy analyses revealed that PD-1 protein is present in virtually all NK cells analyzed. The present findings are compatible with a rapid surface expression of PD-1 in NK cells in response to appropriate, still undefined, stimuli.
U2 - 10.1080/2162402X.2018.1557030
DO - 10.1080/2162402X.2018.1557030
M3 - Article
C2 - 30723590
VL - 8
SP - 1557030
JO - OncoImmunology
JF - OncoImmunology
SN - 2162-4011
IS - 3
ER -