TY - JOUR
T1 - PDMP blocks the BFA-induced ADP-ribosylation of BARS-50 in isolated Golgi membranes
AU - De Matteis, Maria Antonieta
AU - Luna, Ana
AU - Di Tullio, Giuseppe
AU - Corda, Daniela
AU - Kok, Jan Willem
AU - Luini, Alberto
AU - Egea, Gustavo
PY - 1999/10/15
Y1 - 1999/10/15
N2 - We reported that an inhibitor of sphingolipid biosynthesis, D,L-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol (PDMP), blocks brefeldin A (BFA)-induced retrograde membrane transport from the Golgi complex to the endoplasmic reticulum (ER) (Kok et al., 1998, J. Cell Biol. 142, 25-38). We now show that PDMP partially blocks the BFA-induced ADP-ribosylation of the cytosolic protein BARS-50. Moreover, PDMP does not interfere with the BFA-induced inhibition of the binding of ADP-ribosylation factor (ARF) and the coatomer component β-coat protein to Golgi membranes. These results are consistent with a role of ADP-ribosylation in the action of BFA and with the involvement of BARS-50 in the regulation of membrane trafficking. Copyright (C) 1999 Federation of European Biochemical Societies.
AB - We reported that an inhibitor of sphingolipid biosynthesis, D,L-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol (PDMP), blocks brefeldin A (BFA)-induced retrograde membrane transport from the Golgi complex to the endoplasmic reticulum (ER) (Kok et al., 1998, J. Cell Biol. 142, 25-38). We now show that PDMP partially blocks the BFA-induced ADP-ribosylation of the cytosolic protein BARS-50. Moreover, PDMP does not interfere with the BFA-induced inhibition of the binding of ADP-ribosylation factor (ARF) and the coatomer component β-coat protein to Golgi membranes. These results are consistent with a role of ADP-ribosylation in the action of BFA and with the involvement of BARS-50 in the regulation of membrane trafficking. Copyright (C) 1999 Federation of European Biochemical Societies.
KW - ADP-ribosylation
KW - Brefeldin A
KW - D,L-Threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol
KW - Golgi complex
KW - Membrane transport
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U2 - 10.1016/S0014-5793(99)01269-7
DO - 10.1016/S0014-5793(99)01269-7
M3 - Article
C2 - 10526155
AN - SCOPUS:0032832688
VL - 459
SP - 310
EP - 312
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 3
ER -