PDMP blocks the BFA-induced ADP-ribosylation of BARS-50 in isolated Golgi membranes

Maria Antonieta De Matteis; Ana Luna; Giuseppe Di Tullio; Daniela Corda; Jan Willem Kok; Alberto Luini; Gustavo Egea

doi:10.1016/S0014-5793(99)01269-7

PDMP blocks the BFA-induced ADP-ribosylation of BARS-50 in isolated Golgi membranes

Maria Antonieta De Matteis, Ana Luna, Giuseppe Di Tullio, Daniela Corda, Jan Willem Kok, Alberto Luini, Gustavo Egea

IRCCS SDN

Research output: Contribution to journal › Article

Abstract

We reported that an inhibitor of sphingolipid biosynthesis, D,L-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol (PDMP), blocks brefeldin A (BFA)-induced retrograde membrane transport from the Golgi complex to the endoplasmic reticulum (ER) (Kok et al., 1998, J. Cell Biol. 142, 25-38). We now show that PDMP partially blocks the BFA-induced ADP-ribosylation of the cytosolic protein BARS-50. Moreover, PDMP does not interfere with the BFA-induced inhibition of the binding of ADP-ribosylation factor (ARF) and the coatomer component β-coat protein to Golgi membranes. These results are consistent with a role of ADP-ribosylation in the action of BFA and with the involvement of BARS-50 in the regulation of membrane trafficking. Copyright (C) 1999 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	310-312
Number of pages	3
Journal	FEBS Letters
Volume	459
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(99)01269-7
Publication status	Published - Oct 15 1999

Keywords

ADP-ribosylation
Brefeldin A
D,L-Threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol
Golgi complex
Membrane transport

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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De Matteis, M. A., Luna, A., Di Tullio, G., Corda, D., Kok, J. W., Luini, A., & Egea, G. (1999). PDMP blocks the BFA-induced ADP-ribosylation of BARS-50 in isolated Golgi membranes. FEBS Letters, 459(3), 310-312. https://doi.org/10.1016/S0014-5793(99)01269-7

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title = "PDMP blocks the BFA-induced ADP-ribosylation of BARS-50 in isolated Golgi membranes",

abstract = "We reported that an inhibitor of sphingolipid biosynthesis, D,L-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol (PDMP), blocks brefeldin A (BFA)-induced retrograde membrane transport from the Golgi complex to the endoplasmic reticulum (ER) (Kok et al., 1998, J. Cell Biol. 142, 25-38). We now show that PDMP partially blocks the BFA-induced ADP-ribosylation of the cytosolic protein BARS-50. Moreover, PDMP does not interfere with the BFA-induced inhibition of the binding of ADP-ribosylation factor (ARF) and the coatomer component β-coat protein to Golgi membranes. These results are consistent with a role of ADP-ribosylation in the action of BFA and with the involvement of BARS-50 in the regulation of membrane trafficking. Copyright (C) 1999 Federation of European Biochemical Societies.",

keywords = "ADP-ribosylation, Brefeldin A, D,L-Threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, Golgi complex, Membrane transport",

author = "{De Matteis}, {Maria Antonieta} and Ana Luna and {Di Tullio}, Giuseppe and Daniela Corda and Kok, {Jan Willem} and Alberto Luini and Gustavo Egea",

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AU - De Matteis, Maria Antonieta

AU - Luna, Ana

AU - Di Tullio, Giuseppe

AU - Corda, Daniela

AU - Kok, Jan Willem

AU - Luini, Alberto

AU - Egea, Gustavo

PY - 1999/10/15

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N2 - We reported that an inhibitor of sphingolipid biosynthesis, D,L-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol (PDMP), blocks brefeldin A (BFA)-induced retrograde membrane transport from the Golgi complex to the endoplasmic reticulum (ER) (Kok et al., 1998, J. Cell Biol. 142, 25-38). We now show that PDMP partially blocks the BFA-induced ADP-ribosylation of the cytosolic protein BARS-50. Moreover, PDMP does not interfere with the BFA-induced inhibition of the binding of ADP-ribosylation factor (ARF) and the coatomer component β-coat protein to Golgi membranes. These results are consistent with a role of ADP-ribosylation in the action of BFA and with the involvement of BARS-50 in the regulation of membrane trafficking. Copyright (C) 1999 Federation of European Biochemical Societies.

AB - We reported that an inhibitor of sphingolipid biosynthesis, D,L-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol (PDMP), blocks brefeldin A (BFA)-induced retrograde membrane transport from the Golgi complex to the endoplasmic reticulum (ER) (Kok et al., 1998, J. Cell Biol. 142, 25-38). We now show that PDMP partially blocks the BFA-induced ADP-ribosylation of the cytosolic protein BARS-50. Moreover, PDMP does not interfere with the BFA-induced inhibition of the binding of ADP-ribosylation factor (ARF) and the coatomer component β-coat protein to Golgi membranes. These results are consistent with a role of ADP-ribosylation in the action of BFA and with the involvement of BARS-50 in the regulation of membrane trafficking. Copyright (C) 1999 Federation of European Biochemical Societies.

KW - ADP-ribosylation

KW - Brefeldin A

KW - D,L-Threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol

KW - Golgi complex

KW - Membrane transport

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