PEGFR-Tyr 845 expression as prognostic factors in oral squamous cell carcinoma: A tissue-microarray study with clinic-pathological correlations

Gabriella Aquino, Giuseppe Pannone, Angela Santoro, Giuseppina Liguori, Renato Franco, Rosario Serpico, Gianluca Florio, Alfredo De Rosa, Marilena Mattoni, Valentina Cozza, Gerardo Botti, Simona Losito, Francesco Longo, Stefania Staibano, Giovanni Cuda, Lorenzo Lo Muzio, Carolina Sbordone, Pantaleo Bufo, Anna Grimaldi, Michele CaragliaMarina Di Domenico

Research output: Contribution to journalArticlepeer-review

Abstract

The EGFR (epidermal growth factor receptor) a member of the family of transmembrane protein kinase receptors known as the erbB family shows a significant correlation with the presence of metastases and poorly differentiated oral cancer. Aim of the present work is to define the key-role of EGFR in oral cancer prognosis. We have analyzed the EGFR expression on 149 cases of oral squamous cell cancers (OSCC) and we have found that it was poorly expressed in normal oral epithelium, but its expression was significantly increased in OSCCs. Moreover, we have recorded that both pEGFR-Tyr 845 and pEGFR-Tyr 1068 were mainly distributed in high histological grading and in advanced stages. Western blotting has confirmed the total absence of EGFR phosphorylation in normal oral epithelium and the higher level of protein phosphorylation in representative cases of OSCCs. The EGF-R amplification was found by fluorescence in situ hybridization (FISH) in 14% of OSCC; interestingly, EGF-R amplification was mainly observed in OSCC with higher histological grading (G2 and G3) and advanced stage (pT4) sub-groups. Kaplan-Meyer survival analysis suggested that patients with positive pEGFR-Tyr 845 tumors had a worse prognosis and were bad responders to chemotherapy. These results confirm the central role of EGF-R activation status as a prognostic biomarker in OSCC.

Original languageEnglish
Pages (from-to)967-977
Number of pages11
JournalCancer Biology and Therapy
Volume13
Issue number11
DOIs
Publication statusPublished - Sep 2012

Keywords

  • EGFR
  • OSCC
  • Phosphorylated EGFR
  • Targeted therapy

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Molecular Medicine
  • Pharmacology

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