Abstract
Background & Aims: The effect of IFN-α therapy on HBV-specific T-cell responses in HBeAg-negative, genotype D, chronic hepatitis B is largely undefined. Understanding to what extent IFN-α can modulate HBV-specific T-cells is important to define strategies to optimize IFN efficacy and to identify immunological parameters to predict response to therapy. Methods: HBV-specific T-cell responses were analyzed longitudinally ex vivo and after expansion in vitro in 15 patients with genotype D, HBeAg-negative chronic hepatitis B treated with peginterferon-α-2a. HBV proteins and synthetic peptides were used to stimulate T-cell responses. Analysis of the CD4 and CD8 T-cell functions was performed by ELISPOT, intracellular cytokine and tetramer staining. The effect of anti-PD-L1 on T-cell functions was also analyzed. Results: Ex vivo IFN-γ production by total HBV-specific T-cells was significantly greater before therapy in patients who showed HBV DNA
| Original language | English |
|---|---|
| Pages (from-to) | 1239-1246 |
| Number of pages | 8 |
| Journal | Journal of Hepatology |
| Volume | 56 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Jun 2012 |
Fingerprint
Keywords
- Adaptive immunity
- Antiviral therapy
- Cytokines
- PD-1
ASJC Scopus subject areas
- Hepatology
Cite this
Peginterferon-α does not improve early peripheral blood HBV-specific T-cell responses in HBeAg-negative chronic hepatitis. / Penna, Amalia; Laccabue, Diletta; Libri, Irene; Giuberti, Tiziana; Schivazappa, Simona; Alfieri, Arianna; Mori, Cristina; Canetti, Diana; Lampertico, Pietro; Viganò, Mauro; Colombo, Massimo; Loggi, Elisabetta; Missale, Gabriele; Ferrari, Carlo.
In: Journal of Hepatology, Vol. 56, No. 6, 06.2012, p. 1239-1246.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Peginterferon-α does not improve early peripheral blood HBV-specific T-cell responses in HBeAg-negative chronic hepatitis
AU - Penna, Amalia
AU - Laccabue, Diletta
AU - Libri, Irene
AU - Giuberti, Tiziana
AU - Schivazappa, Simona
AU - Alfieri, Arianna
AU - Mori, Cristina
AU - Canetti, Diana
AU - Lampertico, Pietro
AU - Viganò, Mauro
AU - Colombo, Massimo
AU - Loggi, Elisabetta
AU - Missale, Gabriele
AU - Ferrari, Carlo
PY - 2012/6
Y1 - 2012/6
N2 - Background & Aims: The effect of IFN-α therapy on HBV-specific T-cell responses in HBeAg-negative, genotype D, chronic hepatitis B is largely undefined. Understanding to what extent IFN-α can modulate HBV-specific T-cells is important to define strategies to optimize IFN efficacy and to identify immunological parameters to predict response to therapy. Methods: HBV-specific T-cell responses were analyzed longitudinally ex vivo and after expansion in vitro in 15 patients with genotype D, HBeAg-negative chronic hepatitis B treated with peginterferon-α-2a. HBV proteins and synthetic peptides were used to stimulate T-cell responses. Analysis of the CD4 and CD8 T-cell functions was performed by ELISPOT, intracellular cytokine and tetramer staining. The effect of anti-PD-L1 on T-cell functions was also analyzed. Results: Ex vivo IFN-γ production by total HBV-specific T-cells was significantly greater before therapy in patients who showed HBV DNA
AB - Background & Aims: The effect of IFN-α therapy on HBV-specific T-cell responses in HBeAg-negative, genotype D, chronic hepatitis B is largely undefined. Understanding to what extent IFN-α can modulate HBV-specific T-cells is important to define strategies to optimize IFN efficacy and to identify immunological parameters to predict response to therapy. Methods: HBV-specific T-cell responses were analyzed longitudinally ex vivo and after expansion in vitro in 15 patients with genotype D, HBeAg-negative chronic hepatitis B treated with peginterferon-α-2a. HBV proteins and synthetic peptides were used to stimulate T-cell responses. Analysis of the CD4 and CD8 T-cell functions was performed by ELISPOT, intracellular cytokine and tetramer staining. The effect of anti-PD-L1 on T-cell functions was also analyzed. Results: Ex vivo IFN-γ production by total HBV-specific T-cells was significantly greater before therapy in patients who showed HBV DNA
KW - Adaptive immunity
KW - Antiviral therapy
KW - Cytokines
KW - PD-1
UR - http://www.scopus.com/inward/record.url?scp=84861184620&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84861184620&partnerID=8YFLogxK
U2 - 10.1016/j.jhep.2011.12.032
DO - 10.1016/j.jhep.2011.12.032
M3 - Article
VL - 56
SP - 1239
EP - 1246
JO - Journal of Hepatology
JF - Journal of Hepatology
SN - 0168-8278
IS - 6
ER -