PEGylated lipoplexes: Preparation protocols affecting DNA condensation and cell transfection efficiency

Cecilia Bombelli; Francesca Faggioli; Paola Luciani; Giovanna Mancini; Maria Grazia Sacco

doi:10.1021/jm061260f

PEGylated lipoplexes: Preparation protocols affecting DNA condensation and cell transfection efficiency

Cecilia Bombelli, Francesca Faggioli, Paola Luciani, Giovanna Mancini, Maria Grazia Sacco

Istituto Clinico Humanitas

Research output: Contribution to journal › Article › peer-review

Abstract

The inclusion of poly(ethylene glycol) monolaurate in liposomes formulated with dimyristoyl-sn-glycero-3-phosphocholine and certain cationic gemini surfactants improves their capability of condensing DNA into a ψ phase and transfecting it into cells. Both the condensation, observed by circular dichroism, and the transfection efficiency are strongly effected by the protocol of inclusion of the polymer in the formulations. The highest transfection efficiency is observed in correspondence of the highest extent of DNA condensation.

Original language	English
Pages (from-to)	6274-6278
Number of pages	5
Journal	Journal of Medicinal Chemistry
Volume	50
Issue number	24
DOIs	https://doi.org/10.1021/jm061260f
Publication status	Published - Nov 29 2007

ASJC Scopus subject areas

Organic Chemistry

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10.1021/jm061260f

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abstract = "The inclusion of poly(ethylene glycol) monolaurate in liposomes formulated with dimyristoyl-sn-glycero-3-phosphocholine and certain cationic gemini surfactants improves their capability of condensing DNA into a ψ phase and transfecting it into cells. Both the condensation, observed by circular dichroism, and the transfection efficiency are strongly effected by the protocol of inclusion of the polymer in the formulations. The highest transfection efficiency is observed in correspondence of the highest extent of DNA condensation.",

author = "Cecilia Bombelli and Francesca Faggioli and Paola Luciani and Giovanna Mancini and Sacco, {Maria Grazia}",

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T1 - PEGylated lipoplexes

T2 - Preparation protocols affecting DNA condensation and cell transfection efficiency

AU - Bombelli, Cecilia

AU - Faggioli, Francesca

AU - Luciani, Paola

AU - Mancini, Giovanna

AU - Sacco, Maria Grazia

PY - 2007/11/29

Y1 - 2007/11/29

N2 - The inclusion of poly(ethylene glycol) monolaurate in liposomes formulated with dimyristoyl-sn-glycero-3-phosphocholine and certain cationic gemini surfactants improves their capability of condensing DNA into a ψ phase and transfecting it into cells. Both the condensation, observed by circular dichroism, and the transfection efficiency are strongly effected by the protocol of inclusion of the polymer in the formulations. The highest transfection efficiency is observed in correspondence of the highest extent of DNA condensation.

AB - The inclusion of poly(ethylene glycol) monolaurate in liposomes formulated with dimyristoyl-sn-glycero-3-phosphocholine and certain cationic gemini surfactants improves their capability of condensing DNA into a ψ phase and transfecting it into cells. Both the condensation, observed by circular dichroism, and the transfection efficiency are strongly effected by the protocol of inclusion of the polymer in the formulations. The highest transfection efficiency is observed in correspondence of the highest extent of DNA condensation.

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PEGylated lipoplexes: Preparation protocols affecting DNA condensation and cell transfection efficiency

Abstract

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