PEGylated lipoplexes: Preparation protocols affecting DNA condensation and cell transfection efficiency

Cecilia Bombelli, Francesca Faggioli, Paola Luciani, Giovanna Mancini, Maria Grazia Sacco

Research output: Contribution to journalArticlepeer-review

Abstract

The inclusion of poly(ethylene glycol) monolaurate in liposomes formulated with dimyristoyl-sn-glycero-3-phosphocholine and certain cationic gemini surfactants improves their capability of condensing DNA into a ψ phase and transfecting it into cells. Both the condensation, observed by circular dichroism, and the transfection efficiency are strongly effected by the protocol of inclusion of the polymer in the formulations. The highest transfection efficiency is observed in correspondence of the highest extent of DNA condensation.

Original languageEnglish
Pages (from-to)6274-6278
Number of pages5
JournalJournal of Medicinal Chemistry
Volume50
Issue number24
DOIs
Publication statusPublished - Nov 29 2007

ASJC Scopus subject areas

  • Organic Chemistry

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