Pentraxin-3 is upregulated in the central nervous system during MS and EAE, but does not modulate experimental neurological disease

Kimberley Ummenthum, Laura A N Peferoen, Annamaria Finardi, David Baker, Gareth Pryce, Alberto Mantovani, Malika Bsibsi, Barbara Bottazzi, Regina Peferoen-Baert, Paul Van-Der-Valk, Cecilia Garlanda, Markus Kipp, Roberto Furlan, Johannes M. Van Noort, Sandra Amor

Research output: Contribution to journalArticle

Abstract

Pentraxin-3 (PTX3), an acute-phase protein released during inflammation, aids phagocytic clearance of pathogens and apoptotic cells, and plays diverse immunoregulatory roles in tissue injury. In neuroinflammatory diseases, like MS, resident microglia could become activated by endogenous agonists for Toll like receptors (TLRs). Previously we showed a strong TLR2-mediated induction of PTX3 in cultured human microglia and macrophages by HspB5, which accumulates in glia during MS. Given the anti-inflammatory effects of HspB5, we examined the contribution of PTX3 to these effects in MS and its animal model EAE. Our data indicate that TLR engagement effectively induces PTX3 expression in human microglia, and that such expression is readily detectable in MS lesions. Enhanced PTX3 expression is prominently expressed in microglia in preactive MS lesions, and in microglia/macrophages engaged in myelin phagocytosis in actively demyelinating lesions. Yet, we did not detect PTX3 in cerebrospinal fluid of MS patients. PTX3 expression is also elevated in spinal cords during chronic relapsing EAE in Biozzi ABH mice, but the EAE severity and time course in PTX3-deficient mice did not differ from WT mice. Moreover, systemic PTX3 administration did not alter the disease onset or severity. Our findings reveal local functions of PTX3 during neuroinflammation in facilitating myelin phagocytosis, but do not point to a role for PTX3 in controlling the development of autoimmune neuroinflammation. In neuroinflammatory diseases, like multiple sclerosis, resident brain microglia become activated by endogenous agonists for Toll-like receptors (TLRs). These induce secretion of large amounts of pentraxin-3 (PTX3), an antibody-like protein. In this study, we found that PTX3 likely fulfils only local functions and does not inhibit main pathways in experimental autoimmune neuroinflammation (EAE).

Original languageEnglish
Pages (from-to)701-711
Number of pages11
JournalEuropean Journal of Immunology
Volume46
Issue number3
DOIs
Publication statusPublished - Mar 1 2016

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Central Nervous System
Microglia
Toll-Like Receptors
Myelin Sheath
Phagocytosis
PTX3 protein
Biozzi Mice
Macrophages
Acute-Phase Proteins
Neuroglia
Multiple Sclerosis
Cerebrospinal Fluid
Spinal Cord
Anti-Inflammatory Agents
Animal Models
Inflammation
Antibodies
Wounds and Injuries
Brain

Keywords

  • Experimental autoimmune encephalomyelitis (EAE)
  • Innate immunity
  • Microglia
  • Multiple Sclerosis (MS)
  • Pentraxin-3 (PTX3)

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Pentraxin-3 is upregulated in the central nervous system during MS and EAE, but does not modulate experimental neurological disease. / Ummenthum, Kimberley; Peferoen, Laura A N; Finardi, Annamaria; Baker, David; Pryce, Gareth; Mantovani, Alberto; Bsibsi, Malika; Bottazzi, Barbara; Peferoen-Baert, Regina; Van-Der-Valk, Paul; Garlanda, Cecilia; Kipp, Markus; Furlan, Roberto; Van Noort, Johannes M.; Amor, Sandra.

In: European Journal of Immunology, Vol. 46, No. 3, 01.03.2016, p. 701-711.

Research output: Contribution to journalArticle

Ummenthum, K, Peferoen, LAN, Finardi, A, Baker, D, Pryce, G, Mantovani, A, Bsibsi, M, Bottazzi, B, Peferoen-Baert, R, Van-Der-Valk, P, Garlanda, C, Kipp, M, Furlan, R, Van Noort, JM & Amor, S 2016, 'Pentraxin-3 is upregulated in the central nervous system during MS and EAE, but does not modulate experimental neurological disease', European Journal of Immunology, vol. 46, no. 3, pp. 701-711. https://doi.org/10.1002/eji.201545950
Ummenthum, Kimberley ; Peferoen, Laura A N ; Finardi, Annamaria ; Baker, David ; Pryce, Gareth ; Mantovani, Alberto ; Bsibsi, Malika ; Bottazzi, Barbara ; Peferoen-Baert, Regina ; Van-Der-Valk, Paul ; Garlanda, Cecilia ; Kipp, Markus ; Furlan, Roberto ; Van Noort, Johannes M. ; Amor, Sandra. / Pentraxin-3 is upregulated in the central nervous system during MS and EAE, but does not modulate experimental neurological disease. In: European Journal of Immunology. 2016 ; Vol. 46, No. 3. pp. 701-711.
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AU - Bottazzi, Barbara

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AU - Van-Der-Valk, Paul

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