TY - JOUR
T1 - Peptide-Enriched Silk Fibroin Sponge and Trabecular Titanium Composites to Enhance Bone Ingrowth of Prosthetic Implants in an Ovine Model of Bone Gaps
AU - Lovati, Arianna B.
AU - Lopa, Silvia
AU - Bottagisio, Marta
AU - Talò, Giuseppe
AU - Canciani, Elena
AU - Dellavia, Claudia
AU - Alessandrino, Antonio
AU - Biagiotti, Marco
AU - Freddi, Giuliano
AU - Segatti, Francesco
AU - Moretti, Matteo
N1 - Funding Information:
The authors thank Lima Corporate S.p.A. for providing the titanium customized implants. They also thank Dr. Pavel Zh??al, Ph.D. at the Research Centre Rez, ?e? (Czechia) for supporting us with BSE-SEM analyses. Funding. The authors declare that this study received funding from the Italian Ministry of Health [grant number: RF-2010-2322039].
Publisher Copyright:
© Copyright © 2020 Lovati, Lopa, Bottagisio, Talò, Canciani, Dellavia, Alessandrino, Biagiotti, Freddi, Segatti and Moretti.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/10/19
Y1 - 2020/10/19
N2 - Osteoarthritis frequently requires arthroplasty. Cementless implants are widely used in clinics to replace damaged cartilage or missing bone tissue. In cementless arthroplasty, the risk of aseptic loosening strictly depends on implant stability and bone–implant interface, which are fundamental to guarantee the long-term success of the implant. Ameliorating the features of prosthetic materials, including their porosity and/or geometry, and identifying osteoconductive and/or osteoinductive coatings of implant surfaces are the main strategies to enhance the bone-implant contact surface area. Herein, the development of a novel composite consisting in the association of macro-porous trabecular titanium with silk fibroin (SF) sponges enriched with anionic fibroin-derived polypeptides is described. This composite is applied to improve early bone ingrowth into the implant mesh in a sheep model of bone defects. The composite enables to nucleate carbonated hydroxyapatite and accelerates the osteoblastic differentiation of resident cells, inducing an outward bone growth, a feature that can be particularly relevant when applying these implants in the case of poor osseointegration. Moreover, the osteoconductive properties of peptide-enriched SF sponges support an inward bone deposition from the native bone towards the implants. This technology can be exploited to improve the biological functionality of various prosthetic materials in terms of early bone fixation and prevention of aseptic loosening in prosthetic surgery.
AB - Osteoarthritis frequently requires arthroplasty. Cementless implants are widely used in clinics to replace damaged cartilage or missing bone tissue. In cementless arthroplasty, the risk of aseptic loosening strictly depends on implant stability and bone–implant interface, which are fundamental to guarantee the long-term success of the implant. Ameliorating the features of prosthetic materials, including their porosity and/or geometry, and identifying osteoconductive and/or osteoinductive coatings of implant surfaces are the main strategies to enhance the bone-implant contact surface area. Herein, the development of a novel composite consisting in the association of macro-porous trabecular titanium with silk fibroin (SF) sponges enriched with anionic fibroin-derived polypeptides is described. This composite is applied to improve early bone ingrowth into the implant mesh in a sheep model of bone defects. The composite enables to nucleate carbonated hydroxyapatite and accelerates the osteoblastic differentiation of resident cells, inducing an outward bone growth, a feature that can be particularly relevant when applying these implants in the case of poor osseointegration. Moreover, the osteoconductive properties of peptide-enriched SF sponges support an inward bone deposition from the native bone towards the implants. This technology can be exploited to improve the biological functionality of various prosthetic materials in terms of early bone fixation and prevention of aseptic loosening in prosthetic surgery.
KW - bone
KW - osseointegration
KW - prosthetic implant
KW - silk fibroin
KW - titanium
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U2 - 10.3389/fbioe.2020.563203
DO - 10.3389/fbioe.2020.563203
M3 - Article
AN - SCOPUS:85094883653
VL - 8
JO - Frontiers in Bioengineering and Biotechnology
JF - Frontiers in Bioengineering and Biotechnology
SN - 2296-4185
M1 - 563203
ER -