Peptide profiling in epithelial tumor plasma by the emerging proteomic techniques

Emilia Caputo, Maria Luisa Lombardi, Vincenza Luongo, Ramy Moharram, Pete Tornatore, Giuseppe Pirozzi, John Guardiola, Brian M. Martin

Research output: Contribution to journalArticlepeer-review

Abstract

The plasma peptide component (PPC) from ten melanoma (Mel), breast cancer (BC) and healthy individuals was examined by a combination of RP-HPLC, surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) and tandem mass spectrometry. A three peak pattern (2023, 2039, 2053.5 m/z) was primarily observed in melanoma. Two peaks (2236.1 and of 2356.3 m/z) were found only in BC samples. Fibrinogen alpha and inter-α-trypsin inhibitor heavy chain H4 fragments were absent in both tumor samples.

Original languageEnglish
Pages (from-to)59-66
Number of pages8
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume819
Issue number1
DOIs
Publication statusPublished - May 5 2005

Keywords

  • Breast cancer
  • Melanoma
  • Plasma peptide component
  • SELDI-TOF MS

ASJC Scopus subject areas

  • Biochemistry

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