Peptide profiling in epithelial tumor plasma by the emerging proteomic techniques

Emilia Caputo, Maria Luisa Lombardi, Vincenza Luongo, Ramy Moharram, Pete Tornatore, Giuseppe Pirozzi, John Guardiola, Brian M. Martin

Research output: Contribution to journalArticle

Abstract

The plasma peptide component (PPC) from ten melanoma (Mel), breast cancer (BC) and healthy individuals was examined by a combination of RP-HPLC, surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) and tandem mass spectrometry. A three peak pattern (2023, 2039, 2053.5 m/z) was primarily observed in melanoma. Two peaks (2236.1 and of 2356.3 m/z) were found only in BC samples. Fibrinogen alpha and inter-α-trypsin inhibitor heavy chain H4 fragments were absent in both tumor samples.

Original languageEnglish
Pages (from-to)59-66
Number of pages8
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume819
Issue number1
DOIs
Publication statusPublished - May 5 2005

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Tandem Mass Spectrometry
Proteomics
Mass spectrometry
Tumors
Melanoma
Breast Neoplasms
Plasmas
Peptides
Fibrinogen
Ionization
Desorption
Neoplasms
Lasers
High Pressure Liquid Chromatography
inter-alpha-inhibitor

Keywords

  • Breast cancer
  • Melanoma
  • Plasma peptide component
  • SELDI-TOF MS

ASJC Scopus subject areas

  • Biochemistry

Cite this

Peptide profiling in epithelial tumor plasma by the emerging proteomic techniques. / Caputo, Emilia; Lombardi, Maria Luisa; Luongo, Vincenza; Moharram, Ramy; Tornatore, Pete; Pirozzi, Giuseppe; Guardiola, John; Martin, Brian M.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 819, No. 1, 05.05.2005, p. 59-66.

Research output: Contribution to journalArticle

Caputo, Emilia ; Lombardi, Maria Luisa ; Luongo, Vincenza ; Moharram, Ramy ; Tornatore, Pete ; Pirozzi, Giuseppe ; Guardiola, John ; Martin, Brian M. / Peptide profiling in epithelial tumor plasma by the emerging proteomic techniques. In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2005 ; Vol. 819, No. 1. pp. 59-66.
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