Peptide Trimming for MHC Class I Presentation by Endoplasmic Reticulum Aminopeptidases

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Abstract

Endoplasmic reticulum aminopeptidases ERAP1 and ERAP2 have recently emerged as important players in regulating innate and adaptive immune responses by trimming peptide ligands for MHC class I molecules. Functional polymorphisms in ERAP1 and ERAP2 genes have been associated with predisposition to several diseases including autoimmune diseases, viral infections, and virally induced cancers. In this chapter, we describe two basic methods for monitoring peptide-trimming activity by ER aminopeptidases and screening potential chemical inhibitors.

Original languageEnglish
Pages (from-to)45-57
Number of pages13
JournalMethods in molecular biology (Clifton, N.J.)
Volume1988
DOIs
Publication statusPublished - 2019

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Aminopeptidases
Endoplasmic Reticulum
Peptides
Adaptive Immunity
Virus Diseases
Innate Immunity
Autoimmune Diseases
Ligands
Genes
Neoplasms

Cite this

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title = "Peptide Trimming for MHC Class I Presentation by Endoplasmic Reticulum Aminopeptidases",
abstract = "Endoplasmic reticulum aminopeptidases ERAP1 and ERAP2 have recently emerged as important players in regulating innate and adaptive immune responses by trimming peptide ligands for MHC class I molecules. Functional polymorphisms in ERAP1 and ERAP2 genes have been associated with predisposition to several diseases including autoimmune diseases, viral infections, and virally induced cancers. In this chapter, we describe two basic methods for monitoring peptide-trimming activity by ER aminopeptidases and screening potential chemical inhibitors.",
author = "Mirco Compagnone and Doriana Fruci",
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AB - Endoplasmic reticulum aminopeptidases ERAP1 and ERAP2 have recently emerged as important players in regulating innate and adaptive immune responses by trimming peptide ligands for MHC class I molecules. Functional polymorphisms in ERAP1 and ERAP2 genes have been associated with predisposition to several diseases including autoimmune diseases, viral infections, and virally induced cancers. In this chapter, we describe two basic methods for monitoring peptide-trimming activity by ER aminopeptidases and screening potential chemical inhibitors.

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