Performance evaluation of the Artus hepatitis C virus QS-RGQ assay

Pierpaolo Paba, Lavinia Fabeni, Carlo Federico Perno, Marco Ciotti

Research output: Contribution to journalArticle

Abstract

Accurate determination of hepatitis C virus RNA level is essential for evaluating the response to antiviral therapy, to determine the duration of treatment, and to predict treatment outcome. Currently, two real-time based polymerase chain reaction assays are used widely to monitor the hepatitis C RNA level: the Abbott RealTime HCV assay and the Cobas Taqman HCV assay. Recently, a third assay has become commercially available: the Artus HCV QS-RGQ assay, which uses the QIAsymphony SP/AS platform for sample preparation and PCR-setup, and the Rotor-Gene Q for amplification and detection. In this study, the performance of the Artus HCV QS-RGQ assay was tested on 105 plasma samples and compared to that of the Cobas Taqman HCV assay. Linear regression analysis showed a good agreement between the two assays. A slightly better sensitivity was observed with the Cobas Taqman assay, while higher hepatitis C viral RNA levels were measured by the Artus HCV QS-RGQ assay in samples positive for hepatitis C genotypes 4. Taken together, the data suggest that the Artus HCV QS-RGQ assay is useful in a diagnostic setting. The combination with the versatile QIAsymphony SP/AS system may represent a major advantage for clinical virological laboratories aiming at optimizing their workflow.

Original languageEnglish
Pages (from-to)77-80
Number of pages4
JournalJournal of Virological Methods
Volume179
Issue number1
DOIs
Publication statusPublished - Jan 2012

Keywords

  • HCV real-time PCR
  • HCV viral load

ASJC Scopus subject areas

  • Virology

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