TY - JOUR
T1 - Performance evaluation of the automated nucleated red blood cell count of five commercial hematological analyzers
AU - Da Rin, G.
AU - Vidali, M.
AU - Balboni, F.
AU - Benegiamo, A.
AU - Borin, M.
AU - Ciardelli, M. L.
AU - Dima, F.
AU - Di Fabio, A.
AU - Fanelli, A.
AU - Fiorini, F.
AU - Francione, S.
AU - Germagnoli, L.
AU - Gioia, M.
AU - Lari, T.
AU - Lorubbio, M.
AU - Marini, A.
AU - Papa, A.
AU - Seghezzi, M.
AU - Solarino, L.
AU - Pipitone, S.
AU - Tilocca, E.
AU - Buoro, S.
PY - 2017/12/1
Y1 - 2017/12/1
N2 - Introduction: Recent automated hematology analyzers (HAs) can identify and report nucleated red blood cells (NRBC) count as a separate population out of white blood cells (WBC). The aim of this study was to investigate the analytical performances of NRBC enumeration on five top of the range HAs. Methods: We evaluated the within-run and between-day precision, limit of blank (LoB), limit of detection (LoD), and limit of quantitation (LoQ) of XE-2100 and XN-module (Sysmex), ADVIA 2120i (Siemens), BC-6800 (Mindray), and UniCel DxH 800 (Beckman Coulter). Automated NRBC counts were also compared with optical microscopy (OM). Results: The limits of detection for NRBC of the BC-6800, XN-module, XE-2100, UniCel DxH 800, and ADVIA 2120i are 0.035×109/L, 0.019×109/L, 0.067×109/L, 0.038×109/L, and 0.167×109/L, respectively. Our data indicated excellent performance in terms of precision. The agreement with OM was excellent for BC-6800, XN-module, and XE-2100 (Bias 0.023, 0.019, and 0.033×109/L, respectively). ADVIA 2120i displayed a significant constant error and UniCel DxH 800 both proportional and small constant error. Conclusion: Regards to NRBC counting, the performances shown by BC-6800, XN-module, and XE-2100 are excellent also a low count, ADVIA 2120i and UniCel DxH 800 need to be improved.
AB - Introduction: Recent automated hematology analyzers (HAs) can identify and report nucleated red blood cells (NRBC) count as a separate population out of white blood cells (WBC). The aim of this study was to investigate the analytical performances of NRBC enumeration on five top of the range HAs. Methods: We evaluated the within-run and between-day precision, limit of blank (LoB), limit of detection (LoD), and limit of quantitation (LoQ) of XE-2100 and XN-module (Sysmex), ADVIA 2120i (Siemens), BC-6800 (Mindray), and UniCel DxH 800 (Beckman Coulter). Automated NRBC counts were also compared with optical microscopy (OM). Results: The limits of detection for NRBC of the BC-6800, XN-module, XE-2100, UniCel DxH 800, and ADVIA 2120i are 0.035×109/L, 0.019×109/L, 0.067×109/L, 0.038×109/L, and 0.167×109/L, respectively. Our data indicated excellent performance in terms of precision. The agreement with OM was excellent for BC-6800, XN-module, and XE-2100 (Bias 0.023, 0.019, and 0.033×109/L, respectively). ADVIA 2120i displayed a significant constant error and UniCel DxH 800 both proportional and small constant error. Conclusion: Regards to NRBC counting, the performances shown by BC-6800, XN-module, and XE-2100 are excellent also a low count, ADVIA 2120i and UniCel DxH 800 need to be improved.
KW - automated counting
KW - blood cell count
KW - erythroblasts
KW - laboratory hematology
KW - nucleated red blood cells
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U2 - 10.1111/ijlh.12722
DO - 10.1111/ijlh.12722
M3 - Article
C2 - 28990291
AN - SCOPUS:85030677447
VL - 39
SP - 663
EP - 670
JO - International Journal of Laboratory Hematology
JF - International Journal of Laboratory Hematology
SN - 1751-5521
IS - 6
ER -