Performance of a series of novel N-substituted acrylamides in capillary electrophoresis of DNA fragments

Ernesto Simò-Alfonso, Cecilia Gelfi, Mara Lucisano, Pier Giorgio Righetti

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

DNA separations by capillary electrophoresis in viscous solutions of novel polymers, made with Ω-hydroxyl, N-substituted acrylamides (notably N-acryloyl amino propanol, AAP and N-acryloyl amino butanol, AAB) are evaluated. Whereas in standard poly(acrylamide), at 6% concentration, the theoretical plate number (N) does not exceed 500 000, in 6% poly(AAP) N reaches 922 000 and in 6% poly(AAB) N values as high as 1 200 000 are obtained. Also, copolymers of AAP and AAB give N values in excess of 1 million plates. The two novel monomers (AAP and AAB) remain extremely stable during alkaline hydrolysis and display very good hydrophilicity, while being devoid of the noxious habit of auto-polymerization and auto-reticulation exhibited by the previous monomer of this series (N-acryloyl amino ethoxy ethanol). The reasons for such a good performance of the Ω-substituted acrylamide derivatives could be that their polymers may form hydrogen bonds via their distal -OH group during DNA separation.

Original languageEnglish
Pages (from-to)255-261
Number of pages7
JournalJournal of Chromatography A
Volume756
Issue number1-2
DOIs
Publication statusPublished - Dec 20 1996

Fingerprint

Acrylamides
Capillary electrophoresis
Capillary Electrophoresis
Acrylamide
DNA
Polymers
Monomers
1-Propanol
Butanols
Hydrophilicity
Polyacrylates
Hydrophobic and Hydrophilic Interactions
Polymerization
Hydroxyl Radical
Habits
Hydrogen
Hydrolysis
Hydrogen bonds
Ethanol
Copolymers

Keywords

  • Acrylamides
  • DNA
  • N-acryloylaminopropanol
  • N-substituted

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Performance of a series of novel N-substituted acrylamides in capillary electrophoresis of DNA fragments. / Simò-Alfonso, Ernesto; Gelfi, Cecilia; Lucisano, Mara; Righetti, Pier Giorgio.

In: Journal of Chromatography A, Vol. 756, No. 1-2, 20.12.1996, p. 255-261.

Research output: Contribution to journalArticle

Simò-Alfonso, Ernesto ; Gelfi, Cecilia ; Lucisano, Mara ; Righetti, Pier Giorgio. / Performance of a series of novel N-substituted acrylamides in capillary electrophoresis of DNA fragments. In: Journal of Chromatography A. 1996 ; Vol. 756, No. 1-2. pp. 255-261.
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