Abstract
DNA separations by capillary electrophoresis in viscous solutions of novel polymers, made with Ω-hydroxyl, N-substituted acrylamides (notably N-acryloyl amino propanol, AAP and N-acryloyl amino butanol, AAB) are evaluated. Whereas in standard poly(acrylamide), at 6% concentration, the theoretical plate number (N) does not exceed 500 000, in 6% poly(AAP) N reaches 922 000 and in 6% poly(AAB) N values as high as 1 200 000 are obtained. Also, copolymers of AAP and AAB give N values in excess of 1 million plates. The two novel monomers (AAP and AAB) remain extremely stable during alkaline hydrolysis and display very good hydrophilicity, while being devoid of the noxious habit of auto-polymerization and auto-reticulation exhibited by the previous monomer of this series (N-acryloyl amino ethoxy ethanol). The reasons for such a good performance of the Ω-substituted acrylamide derivatives could be that their polymers may form hydrogen bonds via their distal -OH group during DNA separation.
| Original language | English |
|---|---|
| Pages (from-to) | 255-261 |
| Number of pages | 7 |
| Journal | Journal of Chromatography A |
| Volume | 756 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - Dec 20 1996 |
Fingerprint
Keywords
- Acrylamides
- DNA
- N-acryloylaminopropanol
- N-substituted
ASJC Scopus subject areas
- Analytical Chemistry
Cite this
Performance of a series of novel N-substituted acrylamides in capillary electrophoresis of DNA fragments. / Simò-Alfonso, Ernesto; Gelfi, Cecilia; Lucisano, Mara; Righetti, Pier Giorgio.
In: Journal of Chromatography A, Vol. 756, No. 1-2, 20.12.1996, p. 255-261.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Performance of a series of novel N-substituted acrylamides in capillary electrophoresis of DNA fragments
AU - Simò-Alfonso, Ernesto
AU - Gelfi, Cecilia
AU - Lucisano, Mara
AU - Righetti, Pier Giorgio
PY - 1996/12/20
Y1 - 1996/12/20
N2 - DNA separations by capillary electrophoresis in viscous solutions of novel polymers, made with Ω-hydroxyl, N-substituted acrylamides (notably N-acryloyl amino propanol, AAP and N-acryloyl amino butanol, AAB) are evaluated. Whereas in standard poly(acrylamide), at 6% concentration, the theoretical plate number (N) does not exceed 500 000, in 6% poly(AAP) N reaches 922 000 and in 6% poly(AAB) N values as high as 1 200 000 are obtained. Also, copolymers of AAP and AAB give N values in excess of 1 million plates. The two novel monomers (AAP and AAB) remain extremely stable during alkaline hydrolysis and display very good hydrophilicity, while being devoid of the noxious habit of auto-polymerization and auto-reticulation exhibited by the previous monomer of this series (N-acryloyl amino ethoxy ethanol). The reasons for such a good performance of the Ω-substituted acrylamide derivatives could be that their polymers may form hydrogen bonds via their distal -OH group during DNA separation.
AB - DNA separations by capillary electrophoresis in viscous solutions of novel polymers, made with Ω-hydroxyl, N-substituted acrylamides (notably N-acryloyl amino propanol, AAP and N-acryloyl amino butanol, AAB) are evaluated. Whereas in standard poly(acrylamide), at 6% concentration, the theoretical plate number (N) does not exceed 500 000, in 6% poly(AAP) N reaches 922 000 and in 6% poly(AAB) N values as high as 1 200 000 are obtained. Also, copolymers of AAP and AAB give N values in excess of 1 million plates. The two novel monomers (AAP and AAB) remain extremely stable during alkaline hydrolysis and display very good hydrophilicity, while being devoid of the noxious habit of auto-polymerization and auto-reticulation exhibited by the previous monomer of this series (N-acryloyl amino ethoxy ethanol). The reasons for such a good performance of the Ω-substituted acrylamide derivatives could be that their polymers may form hydrogen bonds via their distal -OH group during DNA separation.
KW - Acrylamides
KW - DNA
KW - N-acryloylaminopropanol
KW - N-substituted
UR - http://www.scopus.com/inward/record.url?scp=0030596176&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030596176&partnerID=8YFLogxK
U2 - 10.1016/S0021-9673(96)00650-4
DO - 10.1016/S0021-9673(96)00650-4
M3 - Article
AN - SCOPUS:0030596176
VL - 756
SP - 255
EP - 261
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - 1-2
ER -