Performance of the BD MAX™ instrument with Check-Direct CPE real-time PCR for the detection of carbapenemase genes from rectal swabs, in a setting with endemic dissemination of carbapenemase-producing Enterobacteriaceae

Alberto Antonelli, Fabio Arena, Tommaso Giani, Olga Lorenza Colavecchio, Stoyanka Valentinova Valeva, Suzanne Paule, Peter Boleij, Gian Maria Rossolini

Research output: Contribution to journalArticle

Abstract

Carbapenemase-producing Enterobacteriaceae (CPE) represent an increasing public health issue and the early detection of colonization by CPE can help the implementation of infection control measures among inpatients. In this study, BD MAX Check-Direct CPE screen, with two different Master Mixes (BDMix and CPMix), using the automatic BD MAX instrument, was evaluated for the detection of blaKPC, blaOXA-48, blaVIM and blaNDM genes, in comparison to selective broth enrichment and direct culture from rectal swabs. Among a total of 557 rectal swabs samples, 29 (5.2%) tested positive for CPE (23 for blaKPC, 5 for blaVIM and one for blaOXA-48). The sensitivity, specificity, positive and negative likelihood ratios values were 93.1%, 97.3%, 34.5 and 0.07, for BMix, and 100%, 97.1 %, 34.5 and 0 for CPMix, respectively. Five samples were positive with molecular methods only. The turn-around time was reduced from 18-24 hours (direct culture), or 48 h (broth enrichment) to only 3 h.

Original languageEnglish
Pages (from-to)30-34
Number of pages5
JournalDiagnostic Microbiology and Infectious Disease
Volume86
Issue number1
DOIs
Publication statusPublished - Sep 1 2016

Keywords

  • Carbapenemases
  • CPE
  • RT-PCR

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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