Perfusion of isolated rat kidney with Mesenchymal Stromal Cells/Extracellular Vesicles prevents ischaemic injury

Marilena Gregorini, Valeria Corradetti, Eleonora Francesca Pattonieri, Chiara Rocca, Samantha Milanesi, Andrea Peloso, Silvana Canevari, Loris De Cecco, Matteo Dugo, Maria Antonietta Avanzini, Melissa Mantelli, Marcello Maestri, Pasquale Esposito, Stefania Bruno, Carmelo Libetta, Antonio Dal Canton, Teresa Rampino

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Kidney donation after circulatory death (DCD) is a less than ideal option to meet organ shortages. Hypothermic machine perfusion (HMP) with Belzer solution (BS) improves the viability of DCD kidneys, although the graft clinical course remains critical. Mesenchymal stromal cells (MSC) promote tissue repair by releasing extracellular vesicles (EV). We evaluated whether delivering MSC-/MSC-derived EV during HMP protects rat DCD kidneys from ischaemic injury and investigated the underlying pathogenic mechanisms. Warm ischaemic isolated kidneys were cold-perfused (4 hrs) with BS, BS supplemented with MSC or EV. Renal damage was evaluated by histology and renal gene expression by microarray analysis, RT-PCR. Malondialdehyde, lactate, LDH, glucose and pyruvate were measured in the effluent fluid. MSC-/EV-treated kidneys showed significantly less global ischaemic damage. In the MSC/EV groups, there was up-regulation of three genes encoding enzymes known to improve cell energy metabolism and three genes encoding proteins involved in ion membrane transport. In the effluent fluid, lactate, LDH, MDA and glucose were significantly lower and pyruvate higher in MSC/EV kidneys as compared with BS, suggesting the larger use of energy substrates by MSC/EV kidneys. The addition of MSC/EV to BS during HMP protects the kidney from ischaemic injury by preserving the enzymatic machinery essential for cell viability and protects the kidney from reperfusion damage.

Original languageEnglish
Pages (from-to)3381-3393
Number of pages13
JournalJournal of Cellular and Molecular Medicine
Volume21
Issue number12
DOIs
Publication statusPublished - Dec 1 2017

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Mesenchymal Stromal Cells
Perfusion
Kidney
Wounds and Injuries
Pyruvic Acid
Lactic Acid
Extracellular Vesicles
Glucose
Ion Transport
Microarray Analysis
Reperfusion Injury
Malondialdehyde
Energy Metabolism
Cell Survival
Histology
Up-Regulation
University of Wisconsin-lactobionate solution
Transplants
Gene Expression
Polymerase Chain Reaction

Keywords

  • extracellular vesicles
  • ischaemic injury
  • kidney perfusion
  • microarray analysis
  • stem cells

ASJC Scopus subject areas

  • Molecular Medicine
  • Cell Biology

Cite this

Perfusion of isolated rat kidney with Mesenchymal Stromal Cells/Extracellular Vesicles prevents ischaemic injury. / Gregorini, Marilena; Corradetti, Valeria; Pattonieri, Eleonora Francesca; Rocca, Chiara; Milanesi, Samantha; Peloso, Andrea; Canevari, Silvana; De Cecco, Loris; Dugo, Matteo; Avanzini, Maria Antonietta; Mantelli, Melissa; Maestri, Marcello; Esposito, Pasquale; Bruno, Stefania; Libetta, Carmelo; Dal Canton, Antonio; Rampino, Teresa.

In: Journal of Cellular and Molecular Medicine, Vol. 21, No. 12, 01.12.2017, p. 3381-3393.

Research output: Contribution to journalArticle

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AU - Maestri, Marcello

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