Abstract
Glial cells respond to neuronal activation and release neuroactive molecules (termed “gliotransmitters”) that can affect synaptic activity and modulate plasticity. In this study, we used molecular genetic tools, ultra-structural microscopy, and electrophysiology to assess the role of brain-derived neurotrophic factor (BDNF) on cortical gliotransmission in vivo. We find that glial cells recycle BDNF that was previously secreted by neurons as pro-neurotrophin following long-term potentiation (LTP)-inducing electrical stimulation. Upon BDNF glial recycling, we observed tight, temporal, highly localized TrkB phosphorylation on adjacent neurons, a process required to sustain LTP. Engagement of BDNF recycling by astrocytes represents a novel mechanism by which cortical synapses can expand BDNF action and provide synaptic changes that are relevant for the acquisition of new memories. Accordingly, mice deficient in BDNF glial recycling fail to recognize familiar from novel objects, indicating a physiological requirement for this process in memory consolidation.
| Original language | English |
|---|---|
| Pages (from-to) | 873-887 |
| Number of pages | 15 |
| Journal | Neuron |
| Volume | 92 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Nov 23 2016 |
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ASJC Scopus subject areas
- Neuroscience(all)
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Peri-Synaptic Glia Recycles Brain-Derived Neurotrophic Factor for LTP Stabilization and Memory Retention. / Vignoli, Beatrice; Battistini, Giulia; Melani, Riccardo; Blum, Robert; Santi, Spartaco; Berardi, Nicoletta; Canossa, Marco.
In: Neuron, Vol. 92, No. 4, 23.11.2016, p. 873-887.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Peri-Synaptic Glia Recycles Brain-Derived Neurotrophic Factor for LTP Stabilization and Memory Retention
AU - Vignoli, Beatrice
AU - Battistini, Giulia
AU - Melani, Riccardo
AU - Blum, Robert
AU - Santi, Spartaco
AU - Berardi, Nicoletta
AU - Canossa, Marco
PY - 2016/11/23
Y1 - 2016/11/23
N2 - Glial cells respond to neuronal activation and release neuroactive molecules (termed “gliotransmitters”) that can affect synaptic activity and modulate plasticity. In this study, we used molecular genetic tools, ultra-structural microscopy, and electrophysiology to assess the role of brain-derived neurotrophic factor (BDNF) on cortical gliotransmission in vivo. We find that glial cells recycle BDNF that was previously secreted by neurons as pro-neurotrophin following long-term potentiation (LTP)-inducing electrical stimulation. Upon BDNF glial recycling, we observed tight, temporal, highly localized TrkB phosphorylation on adjacent neurons, a process required to sustain LTP. Engagement of BDNF recycling by astrocytes represents a novel mechanism by which cortical synapses can expand BDNF action and provide synaptic changes that are relevant for the acquisition of new memories. Accordingly, mice deficient in BDNF glial recycling fail to recognize familiar from novel objects, indicating a physiological requirement for this process in memory consolidation.
AB - Glial cells respond to neuronal activation and release neuroactive molecules (termed “gliotransmitters”) that can affect synaptic activity and modulate plasticity. In this study, we used molecular genetic tools, ultra-structural microscopy, and electrophysiology to assess the role of brain-derived neurotrophic factor (BDNF) on cortical gliotransmission in vivo. We find that glial cells recycle BDNF that was previously secreted by neurons as pro-neurotrophin following long-term potentiation (LTP)-inducing electrical stimulation. Upon BDNF glial recycling, we observed tight, temporal, highly localized TrkB phosphorylation on adjacent neurons, a process required to sustain LTP. Engagement of BDNF recycling by astrocytes represents a novel mechanism by which cortical synapses can expand BDNF action and provide synaptic changes that are relevant for the acquisition of new memories. Accordingly, mice deficient in BDNF glial recycling fail to recognize familiar from novel objects, indicating a physiological requirement for this process in memory consolidation.
UR - http://www.scopus.com/inward/record.url?scp=84997207195&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84997207195&partnerID=8YFLogxK
U2 - 10.1016/j.neuron.2016.09.031
DO - 10.1016/j.neuron.2016.09.031
M3 - Article
AN - SCOPUS:84997207195
VL - 92
SP - 873
EP - 887
JO - Neuron
JF - Neuron
SN - 0896-6273
IS - 4
ER -