Abstract
Pericellular pO2 (p(pc)O2) was compared with the release of cytoplasmatic enzyme lactate dehydrogenase (LDH) and mitochondrial metabolic function (tetrazolium salt reduction, MTT) as an alternative method to evaluate cytotoxicity. L-929 cells were seeded and incubated with 3 different control materials to test their cytotoxicity effects by these methods. High density polyethylene and copper were respectively used as negative and positive controls, while a 0.9% NaCl solution was as reagent control and extraction vehicle. P(pc)O2 was measured by a rod polarographic Clark-type probe connected to Licox pO2 computer (GMS mbH, Kiel, Germany). One-way ANOVA test showed significant differences among groups regarding every methods (p <0.0005). The comparison of the mean variation coefficients of three methods showed no significant differences. In addition, a significant correlation was found between p(pc)O2 and MTT (r = -0.621; p <0.001) as well as p(pc)O2 and LDH data (r = 0.474; p <0.001). In conclusion, p(pc)O2 may be considered a reliable, safely and alternative method to test cytotoxicity.
Original language | English |
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Pages (from-to) | 579-586 |
Number of pages | 8 |
Journal | Artificial Cells, Blood Substitutes, and Immobilization Biotechnology |
Volume | 24 |
Issue number | 6 |
Publication status | Published - Nov 1996 |
ASJC Scopus subject areas
- Biotechnology
- Biomedical Engineering
- Biomaterials
- Hematology