Perilipin 3 modulates specific lipogenic pathways in SZ95 sebocytes

Emanuela Camera, Maik Dahlhoff, Matteo Ludovici, Christos C. Zouboulis, Marlon R. Schneider

Research output: Contribution to journalArticlepeer-review

Abstract

Lipid droplets (LD) are dynamic organelles that manage cellular lipid synthesis, storage and retrieval. Although LD-associated proteins, including the perilipin family (PLIN1-PLIN5), are essential for these functions, they have been poorly characterized in sebocytes. Here, we employed siRNAs to downregulate PLIN3 in SZ95 sebaceous gland cells and evaluated the consequences in lipid accumulation by nile red staining and mass spectrometry. Nile red staining revealed that siRNA-mediated downregulation of PLIN3 significantly impaired linoleic acid-induced lipid accumulation in SZ95 sebocytes. Mass spectrometry revealed that PLIN3 was implicated in the metabolism of linoleic acid, a lipid source used in the build-up of triglycerides, among other acyl lipids. Furthermore, the expression of key enzymes of sebaceous lipogenesis was altered in PLIN3-deficient sebocytes, consistent with the changes observed in the neutral lipid abundance, suggesting that PLIN3 functions are intertwined with the lipogenic pathways implicated in sebaceous lipogenesis, such as desaturation and triglyceride synthesis.

Original languageEnglish
Pages (from-to)759-761
Number of pages3
JournalExperimental Dermatology
Volume23
Issue number10
DOIs
Publication statusPublished - Oct 1 2014

Keywords

  • Lipid droplets
  • Perilipins
  • Sebaceous glands
  • SZ95 sebocytes

ASJC Scopus subject areas

  • Dermatology
  • Molecular Biology
  • Biochemistry

Fingerprint

Dive into the research topics of 'Perilipin 3 modulates specific lipogenic pathways in SZ95 sebocytes'. Together they form a unique fingerprint.

Cite this