Peripheral blood lymphocytes of patients with common variable immunodeficiency (CVI) produce reduced levels of interleukin-4, interleukin-2 and interferon-gamma, but proliferate normally upon activation by mitogens

G. Pastorelli, M. G. Roncarolo, J. L. Touraine, G. Peronne, P. A. Tovo, J. E. De Vries

Research output: Contribution to journalArticlepeer-review

Abstract

Peripheral blood lymphocytes (PBL) of 11 patients with CVI produced reduced levels of interleukin-4 (IL-4) upon activation by mitogens as compared with those secreted by PBL of healthy donors. The interleukin-2 (IL-2) and interferon-gamma (IFN-γ) production by PBL of a series of 15 patients with CVI was also reduced. Decreased levels of IL-4 or IL-2 and IFN-γ production were not only observed after activation by phytohaemagglutinin (PHA) at concentrations of 10 and 1 μg/ml, but also after activation by concanavalin A (Con A, 10 μg/ml). Longitudinal studies indicated that this defective lymphokine production was consistent upon testing periods up to 5 months. No correlation between reduced IL-4, IL-2 or IFN-γ production was observed. PBL of patients that produced reduced levels of one lymphokine generally secreted normal levels of the other two lymphokines. Despite the reduced synthesis of the T cell growth factors IL-2 and IL-4, the proliferative responses of the PBL of the patients were in the normal range, which is compatible with the finding that IL-2 and IL-4 have synergistic effects on lymphocyte proliferation, particularly when one of these lymphokines is present at suboptimal concentrations. Since IL-2, IL-4 and IFN-γ can act as B cell growth and differentiation factors, our data suggest that the reduced synthesis of these lymphokines may contribute to the deficient immunoglobulin production in patients with CVI.

Original languageEnglish
Pages (from-to)334-340
Number of pages7
JournalClinical and Experimental Immunology
Volume78
Issue number3
Publication statusPublished - 1989

Keywords

  • common variable immunodeficiency
  • interferon-gamma
  • interleukin-2
  • interleukin-4
  • production
  • T cell proliferation

ASJC Scopus subject areas

  • Immunology

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