Peripheral blood progenitor cell mobilization in healthy donors receiving recombinant human granulocyte colony-stimulating factor

Carmelo Carlo-Stella, Clara Cesana, Ester Regazzi, Franca Falzetti, Franco Aversa, Vittorio Rizzoli, Massimo Martelli, Antonio Tabilio

Research output: Contribution to journalArticle

Abstract

Objective. We analyzed the incidence of primitive (LTC-IC) and committed (CFU-mix, BFU-E, CFU-GM) hematopoietic progenitors detected under steady- state conditions and upon progenitor cell mobilization in a cohort of healthy donors receiving recombinant human granulocyte colony-stimulating factor (rhG-CSF). Materials and methods. Healthy donors (n = 30) of HLA-mismatched or -matched stem cell transplants were mobilized with rhG-CSF (8 μg/Kg body weight subcutaneously twice daily until completion of leukapheresis). PBPC collections were started after 4 days of rhG-CSF therapy. Results. Steady- state incidence of bone marrow LTC-IC, but not committed progenitors, significantly correlated with the numbers of mobilized CD34+ cells (r = 0.6, p = 0.004), CFU-GM (r = 0.79, p = 0.0005) and CFC (r = 0.76, p = 0.001) detected after 4 days of rhG-CSF therapy. Statistically significant correlations were also found between steady-state blood CFU-GM and peak numbers of CD341 cells (r = 0.68, p = 0.001), numbers of day 4 CD341 cells (r = 0.52, p = 0.005), CFU-GM (r = 0.63, p = 0.002), and CFC (r = 0.61, p = 0.003). Conclusion. Our data show that in normal volunteers baseline marrow LTC-IC and blood CFU-GM correlate with rhG-CSF-mobilized PBPC. The potential clinical relevance of these findings in the identification of poor mobilizers will be tested in a prospective study. (C) 2000 International Society for Experimental Hematology.

Original languageEnglish
Pages (from-to)216-224
Number of pages9
JournalExperimental Hematology
Volume28
Issue number2
DOIs
Publication statusPublished - Feb 2000

Fingerprint

Granulocyte-Macrophage Progenitor Cells
Granulocyte Colony-Stimulating Factor
Blood Cells
Stem Cells
Tissue Donors
Bone Marrow
Leukapheresis
Erythroid Precursor Cells
Incidence
Healthy Volunteers
Cell Count
Body Weight
Prospective Studies
Transplants
Therapeutics

Keywords

  • CFU-GM
  • LTC-IC
  • Mobilization
  • Peripheral blood progenitor cells
  • Recombinant human granulocyte colony-stimulating factor

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Peripheral blood progenitor cell mobilization in healthy donors receiving recombinant human granulocyte colony-stimulating factor. / Carlo-Stella, Carmelo; Cesana, Clara; Regazzi, Ester; Falzetti, Franca; Aversa, Franco; Rizzoli, Vittorio; Martelli, Massimo; Tabilio, Antonio.

In: Experimental Hematology, Vol. 28, No. 2, 02.2000, p. 216-224.

Research output: Contribution to journalArticle

Carlo-Stella, Carmelo ; Cesana, Clara ; Regazzi, Ester ; Falzetti, Franca ; Aversa, Franco ; Rizzoli, Vittorio ; Martelli, Massimo ; Tabilio, Antonio. / Peripheral blood progenitor cell mobilization in healthy donors receiving recombinant human granulocyte colony-stimulating factor. In: Experimental Hematology. 2000 ; Vol. 28, No. 2. pp. 216-224.
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AU - Carlo-Stella, Carmelo

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AU - Aversa, Franco

AU - Rizzoli, Vittorio

AU - Martelli, Massimo

AU - Tabilio, Antonio

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AB - Objective. We analyzed the incidence of primitive (LTC-IC) and committed (CFU-mix, BFU-E, CFU-GM) hematopoietic progenitors detected under steady- state conditions and upon progenitor cell mobilization in a cohort of healthy donors receiving recombinant human granulocyte colony-stimulating factor (rhG-CSF). Materials and methods. Healthy donors (n = 30) of HLA-mismatched or -matched stem cell transplants were mobilized with rhG-CSF (8 μg/Kg body weight subcutaneously twice daily until completion of leukapheresis). PBPC collections were started after 4 days of rhG-CSF therapy. Results. Steady- state incidence of bone marrow LTC-IC, but not committed progenitors, significantly correlated with the numbers of mobilized CD34+ cells (r = 0.6, p = 0.004), CFU-GM (r = 0.79, p = 0.0005) and CFC (r = 0.76, p = 0.001) detected after 4 days of rhG-CSF therapy. Statistically significant correlations were also found between steady-state blood CFU-GM and peak numbers of CD341 cells (r = 0.68, p = 0.001), numbers of day 4 CD341 cells (r = 0.52, p = 0.005), CFU-GM (r = 0.63, p = 0.002), and CFC (r = 0.61, p = 0.003). Conclusion. Our data show that in normal volunteers baseline marrow LTC-IC and blood CFU-GM correlate with rhG-CSF-mobilized PBPC. The potential clinical relevance of these findings in the identification of poor mobilizers will be tested in a prospective study. (C) 2000 International Society for Experimental Hematology.

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