Peripheral blood stem cell collection and transplantation in paediatric malignancies: a monocentric experience.

M. G. Petris, G. De Silvestro, S. Cesaro, C. Messina, P. Marson, R. Destro, M. V. Gazzola, L. Masiero, M. Carli, S. Varotto, E. Calore, G. Scarzello, L. Zanesco

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Abstract

Thirty-seven patients underwent peripheral blood stem cell (PBSC) collection from May 1994 to May 1997. Twenty-five were males and 12 were females, the median age at collection was 11.5 years (range 1-27.4) and the median weight was 38 kg (range 9-80). As mobilising chemotherapy, cyclophosphamide, etoposide, doxorubicin and cytosine arabinoside were the drugs most frequently used in association with G-CSF for a total of 47 courses. Sixty-one aphereses were performed with a median collection of CD34+ and CFU-GM cells/kg of 3.6 x 10(6) (range 0.6-31.8) and 24.4 x 10(4) (range 0.1-1260), respectively. Minimal residual disease (MRD) was found in five of the 30 investigated aphereses. Twenty-one of the 37 patients underwent high-dose chemotherapy with autologous stem cell rescue: in seven the stem cell source was peripheral blood and bone marrow. The median duration of hospitalization was 18 days for the PBSC group and 23 days for the PBSC/ABMT group. Overall survival was 78.7% at a median follow-up of 18 months (range 2-31) and the DFS was 52% without difference depending on stem cell source. Compared to a historical group of ABMT patients, the PBSC group showed a statistical advantage in terms of neutrophils and platelet engraftment, blood and platelet requirements, and length of hospitalization. PBSC collection is a feasible procedure also in the paediatric setting providing that vascular access is adequate. As already reported, PBSC transplant results in faster engraftment and shorter hospitalization that could allow a better utilization of health financial resources. The question whether the source of stem cells could influence transplant outcome would require a prospective randomised study.

Original languageEnglish
JournalBone Marrow Transplantation
Volume22 Suppl 5
Publication statusPublished - Dec 1998

ASJC Scopus subject areas

  • Hematology
  • Transplantation

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    Petris, M. G., De Silvestro, G., Cesaro, S., Messina, C., Marson, P., Destro, R., Gazzola, M. V., Masiero, L., Carli, M., Varotto, S., Calore, E., Scarzello, G., & Zanesco, L. (1998). Peripheral blood stem cell collection and transplantation in paediatric malignancies: a monocentric experience. Bone Marrow Transplantation, 22 Suppl 5.