Peripheral blood stem cells in children with solid tumors: Part I - Feasibility and application

G. Deb, C. Dominici, A. Angioni, P. Balloni, G. Catena, P. Fidani, L. De Sio, A. Landolfo, A. Amici, L. Helson, A. Donfrancesco

Research output: Contribution to journalArticlepeer-review

Abstract

A method for collecting peripheral blood mononuclear cells following mobilizing chemotherapy in pediatric patients is described. The critical elements of the method included temporary heparinization of the patient to reduce citrate overload, and limiting extracorporeal circulation to 15% of the patients blood volume using packed red blood cells and albumin. A median of 0.9 x 108 mononuclear cells/kg per collection were harvested in 40 collections from eight patients with only one episode of fever and chills. Peripheral blood stem cells were reinfused into six of these patients with refractory/recurrent pediatric tumors after intensive chemotherapy. Bone marrow reconstitution followed with a mean of 30 days (19-38) for absolute neutrophils and 48 days (32-275+) for platelets. Previous chemotherapy did not appear to affect peripheral blood stem cell efficacy in reconstituting chemotherapy-ablated bone marrow.

Original languageEnglish
Pages (from-to)2569-2571
Number of pages3
JournalAnticancer Research
Volume13
Issue number6 B
Publication statusPublished - 1993

Keywords

  • Autologous peripheral blood stem cells
  • Heparin-acid-citrate-dextrose
  • Pediatric apheresis
  • Pediatric tumors
  • Recovery time

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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