Abstract
Molecular monitoring of the BCR-ABL1 transcript in chronic myelogenous leukemia (CML) using quantitative real-time PCR (RQ-PCR) can be performed using either bone marrow (BM) or peripheral blood (PB). However, a recent report by Stock et al. [International Journal of Oncology 28 (2006) 1099] questioned the reliability of PB samples for BCR-ABL1 detection as performed by RQ-PCR. We report a study on 114 CML patients who received allogeneic stem cell transplantation (ASCT), and who were monitored by RQ-PCR using paired samples of BM and PB: the total number of determinations was 428, with a median follow-up after transplant of 8 years. BCR-ABL1 transcript was undetectable or 0.1% in 36 (16.8%) paired determinations and was discordant in 10 (4.7%). Agreement between PB and BM results was quantified by the kappa test (k = 0.85; 95% CI 0.76-0.94). This study shows that BCR-ABL1 RQ-PCR monitoring of CML patients after ASCT with PB is concordant with BM in 95.3% of cases, and thus may be used to monitor the disease. This may be relevant when discussing both quality of life issues and the need for post-transplant monitoring with the patient.
Original language | English |
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Pages (from-to) | 387-391 |
Number of pages | 5 |
Journal | International Journal of Laboratory Hematology |
Volume | 32 |
Issue number | 4 |
DOIs | |
Publication status | Published - Aug 2010 |
Keywords
- Allogeneic stem cell transplantation
- Bone marrow
- Chronic myeloid leukemia
- Peripheral blood
- RQ-PCR
ASJC Scopus subject areas
- Clinical Biochemistry
- Biochemistry, medical
- Hematology