Abstract
Alteration of key regulatory kinases may cause aberrant protein phosphorylation and aggregation in Alzheimer's disease (AD) and Parkinson's disease (PD). In this study, we investigated expression and phosphorylation status of glycogen synthase kinase 3 (GSK-3), protein kinase B (Akt) and tau protein in peripheral blood lymphocytes of 20 AD, 25 PD patients and 20 healthy controls.GSK-3 was increased in AD and PD patients. In these latter, GSK-3 levels were positively correlated with daily l-Dopa intake. Phosphorylated Akt expression was augmented in both groups; total Akt levels were increased only in AD patients and were positively correlated with disease duration and severity. Total and phosphorylated tau were increased only in AD, with phospho-tau levels being positively correlated with levels of total tau, Akt, and disease duration. No correlations between protein levels and clinical variables were found in PD patients.Investigation of peripheral changes in the expression of specific kinases may, therefore, lead to the development of innovative biomarkers of neurodegeneration, particularly for AD.
| Original language | English |
|---|---|
| Pages (from-to) | 2142-2151 |
| Number of pages | 10 |
| Journal | Neurobiology of Aging |
| Volume | 32 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - Dec 2011 |
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Keywords
- Glycogen kinase 3
- Neurodegenerative disease
- Peripheral blood lymphocytes
- Protein kinase B
- Tau
ASJC Scopus subject areas
- Clinical Neurology
- Neuroscience(all)
- Ageing
- Developmental Biology
- Geriatrics and Gerontology
Cite this
Peripheral expression of key regulatory kinases in Alzheimer's disease and Parkinson's disease. / Armentero, M. T.; Sinforiani, E.; Ghezzi, C.; Bazzini, E.; Levandis, G.; Ambrosi, G.; Zangaglia, R.; Pacchetti, C.; Cereda, C.; Cova, E.; Basso, E.; Celi, D.; Martignoni, E.; Nappi, G.; Blandini, F.
In: Neurobiology of Aging, Vol. 32, No. 12, 12.2011, p. 2142-2151.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Peripheral expression of key regulatory kinases in Alzheimer's disease and Parkinson's disease
AU - Armentero, M. T.
AU - Sinforiani, E.
AU - Ghezzi, C.
AU - Bazzini, E.
AU - Levandis, G.
AU - Ambrosi, G.
AU - Zangaglia, R.
AU - Pacchetti, C.
AU - Cereda, C.
AU - Cova, E.
AU - Basso, E.
AU - Celi, D.
AU - Martignoni, E.
AU - Nappi, G.
AU - Blandini, F.
PY - 2011/12
Y1 - 2011/12
N2 - Alteration of key regulatory kinases may cause aberrant protein phosphorylation and aggregation in Alzheimer's disease (AD) and Parkinson's disease (PD). In this study, we investigated expression and phosphorylation status of glycogen synthase kinase 3 (GSK-3), protein kinase B (Akt) and tau protein in peripheral blood lymphocytes of 20 AD, 25 PD patients and 20 healthy controls.GSK-3 was increased in AD and PD patients. In these latter, GSK-3 levels were positively correlated with daily l-Dopa intake. Phosphorylated Akt expression was augmented in both groups; total Akt levels were increased only in AD patients and were positively correlated with disease duration and severity. Total and phosphorylated tau were increased only in AD, with phospho-tau levels being positively correlated with levels of total tau, Akt, and disease duration. No correlations between protein levels and clinical variables were found in PD patients.Investigation of peripheral changes in the expression of specific kinases may, therefore, lead to the development of innovative biomarkers of neurodegeneration, particularly for AD.
AB - Alteration of key regulatory kinases may cause aberrant protein phosphorylation and aggregation in Alzheimer's disease (AD) and Parkinson's disease (PD). In this study, we investigated expression and phosphorylation status of glycogen synthase kinase 3 (GSK-3), protein kinase B (Akt) and tau protein in peripheral blood lymphocytes of 20 AD, 25 PD patients and 20 healthy controls.GSK-3 was increased in AD and PD patients. In these latter, GSK-3 levels were positively correlated with daily l-Dopa intake. Phosphorylated Akt expression was augmented in both groups; total Akt levels were increased only in AD patients and were positively correlated with disease duration and severity. Total and phosphorylated tau were increased only in AD, with phospho-tau levels being positively correlated with levels of total tau, Akt, and disease duration. No correlations between protein levels and clinical variables were found in PD patients.Investigation of peripheral changes in the expression of specific kinases may, therefore, lead to the development of innovative biomarkers of neurodegeneration, particularly for AD.
KW - Glycogen kinase 3
KW - Neurodegenerative disease
KW - Peripheral blood lymphocytes
KW - Protein kinase B
KW - Tau
UR - http://www.scopus.com/inward/record.url?scp=80053625945&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80053625945&partnerID=8YFLogxK
U2 - 10.1016/j.neurobiolaging.2010.01.004
DO - 10.1016/j.neurobiolaging.2010.01.004
M3 - Article
C2 - 20106550
AN - SCOPUS:80053625945
VL - 32
SP - 2142
EP - 2151
JO - Neurobiology of Aging
JF - Neurobiology of Aging
SN - 0197-4580
IS - 12
ER -