Peripheral nerve morphogenesis induced by scaffold micropatterning

Federica Cerri, Luca Salvatore, Danish Memon, Filippo Martinelli Boneschi, Marta Madaghiele, Paola Brambilla, Ubaldo Del Carro, Carla Taveggia, Nilo Riva, Amelia Trimarco, Ignazio D. Lopez, Giancarlo Comi, Stefano Pluchino, Gianvito Martino, Alessandro Sannino, Angelo Quattrini

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Several bioengineering approaches have been proposed for peripheral nervous system repair, with limited results and still open questions about the underlying molecular mechanisms. We assessed the biological processes that occur after the implantation of collagen scaffold with a peculiar porous micro-structure of the wall in a rat sciatic nerve transection model compared to commercial collagen conduits and nerve crush injury using functional, histological and genome wide analyses. We demonstrated that within 60 days, our conduit had been completely substituted by a normal nerve. Gene expression analysis documented a precise sequential regulation of known genes involved in angiogenesis, Schwann cells/axons interactions and myelination, together with a selective modulation of key biological pathways for nerve morphogenesis induced by porous matrices. These data suggest that the scaffold's micro-structure profoundly influences cell behaviors and creates an instructive micro-environment to enhance nerve morphogenesis that can be exploited to improve recovery and understand the molecular differences between repair and regeneration.

Original languageEnglish
Pages (from-to)4035-4045
Number of pages11
JournalBiomaterials
Volume35
Issue number13
DOIs
Publication statusPublished - Apr 2014

Fingerprint

Morphogenesis
Peripheral Nerves
Collagen
Scaffolds
Repair
Genes
Nerve Crush
Biological Phenomena
Bioengineering
Microstructure
Schwann Cells
Peripheral Nervous System
Neurology
Sciatic Nerve
Scaffolds (biology)
Gene expression
Cell Communication
Axons
Rats
Regeneration

Keywords

  • Biomaterials
  • Medical device
  • Nerve regeneration
  • Peripheral nervous system

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Cerri, F., Salvatore, L., Memon, D., Martinelli Boneschi, F., Madaghiele, M., Brambilla, P., ... Quattrini, A. (2014). Peripheral nerve morphogenesis induced by scaffold micropatterning. Biomaterials, 35(13), 4035-4045. https://doi.org/10.1016/j.biomaterials.2014.01.069

Peripheral nerve morphogenesis induced by scaffold micropatterning. / Cerri, Federica; Salvatore, Luca; Memon, Danish; Martinelli Boneschi, Filippo; Madaghiele, Marta; Brambilla, Paola; Del Carro, Ubaldo; Taveggia, Carla; Riva, Nilo; Trimarco, Amelia; Lopez, Ignazio D.; Comi, Giancarlo; Pluchino, Stefano; Martino, Gianvito; Sannino, Alessandro; Quattrini, Angelo.

In: Biomaterials, Vol. 35, No. 13, 04.2014, p. 4035-4045.

Research output: Contribution to journalArticle

Cerri, F, Salvatore, L, Memon, D, Martinelli Boneschi, F, Madaghiele, M, Brambilla, P, Del Carro, U, Taveggia, C, Riva, N, Trimarco, A, Lopez, ID, Comi, G, Pluchino, S, Martino, G, Sannino, A & Quattrini, A 2014, 'Peripheral nerve morphogenesis induced by scaffold micropatterning', Biomaterials, vol. 35, no. 13, pp. 4035-4045. https://doi.org/10.1016/j.biomaterials.2014.01.069
Cerri F, Salvatore L, Memon D, Martinelli Boneschi F, Madaghiele M, Brambilla P et al. Peripheral nerve morphogenesis induced by scaffold micropatterning. Biomaterials. 2014 Apr;35(13):4035-4045. https://doi.org/10.1016/j.biomaterials.2014.01.069
Cerri, Federica ; Salvatore, Luca ; Memon, Danish ; Martinelli Boneschi, Filippo ; Madaghiele, Marta ; Brambilla, Paola ; Del Carro, Ubaldo ; Taveggia, Carla ; Riva, Nilo ; Trimarco, Amelia ; Lopez, Ignazio D. ; Comi, Giancarlo ; Pluchino, Stefano ; Martino, Gianvito ; Sannino, Alessandro ; Quattrini, Angelo. / Peripheral nerve morphogenesis induced by scaffold micropatterning. In: Biomaterials. 2014 ; Vol. 35, No. 13. pp. 4035-4045.
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