Peripheral T cell lymphomas with follicular T helper phenotype: A new basket or a distinct entity? Revising Karl Lennert's personal archive

Claudio Agostinelli, Sylvia Hartmann, Wolfram Klapper, Penelope Korkolopoulou, Simona Righi, Teresa Marafioti, Pier Paolo Piccaluga, Efstratios Patsouris, Martin Leo Hansmann, Karl Lennert, Stefano A. Pileri

Research output: Contribution to journalArticlepeer-review

Abstract

Aims: To revise 25 cases selected from Karl Lennert's personal archive (21) and Bologna and Frankfurt Registries (four) because of cytological similarities. Methods and results: All cases were provided with paraffin blocks and studied by immunohistochemistry and molecular techniques. While phenotyping was very informative, among molecular studies only EBER in situ-hybridization (ISH) was successful. Twenty-two cases were concluded as peripheral T cell lymphomas (PTCL). Of these, six were reclassified as angioimmunoblastic T cell lymphoma (AITL), 13 as PTCL, not otherwise specified (NOS), including four follicular variants and one tumour with T-zone pattern, and three as borderline tumours between AITL and PTCL/NOS. All these cases consisted homogeneously of small/medium-sized elements with mild nuclear atypia and an evident rim of clear/pale cytoplasm. On immunohistochemistry, they regularly expressed three to six follicular helper T cell (FTH)-associated markers. EBER-ISH revealed scattered EBV-infected B cells in all tumours except those with 'follicular' growth pattern. The content of follicular dendritic cells and high-endothelial venules varied significantly depending on the histotype. Conclusions: This study shows that: (i) historical material can be still employed usefully, and (ii) the FTH-phenotype corresponds to a broad spectrum of PTCLs that might form a new category to be validated in future molecular and clinicopathological analyses.

Original languageEnglish
Pages (from-to)679-691
Number of pages13
JournalHistopathology
Volume59
Issue number4
DOIs
Publication statusPublished - Oct 2011

Keywords

  • Clear cell
  • Follicular helper T cell
  • Immunohistochemistry
  • In-situ hybridization
  • Peripheral T cell lymphoma

ASJC Scopus subject areas

  • Histology
  • Pathology and Forensic Medicine

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