Peripheral T cell tolerance occurs early during spontaneous prostate cancer development and can be rescued by dendritic cell immunization

Elena Degl'Innocenti, Matteo Grioni, Andrea Boni, Annalisa Camporeale, Maria T S Bertilaccio, Massimo Freschi, Antonella Monno, Cinzia Arcelloni, Norman M. Greenberg, Matteo Bellone

Research output: Contribution to journalArticle

Abstract

In the tumor-prone transgenic adenocarcinoma mouse prostate (TRAMP) mouse model we followed the fate of the immune response against the SV40 large T antigen (Tag) selectively expressed in the prostate epithelium during the endogenous transformation from normal cells to tumors. Young (5-7-week-old) male TRAMP mice, despite a dim and patchy expression of Tag overlapping foci of mouse prostate intraepithelial neoplasia, displayed a strong Tag-specific cytotoxic T lymphocyte (CTL) response after an intradermal injection of peptide-pulsed dendritic cells (DC). This response was weaker than the one found in vaccinated wild-type littermates, and was characterized by a reduced frequency and avidity of Tag-specific CTL. Early DC vaccination also subverted the profound state of peripheral tolerance typically found in TRAMP mice older than 9-10 weeks. The DC-induced CTL response indeed was still detectable in TRAMP mice of 16 weeks, and was associated with histology evidence of reduced disease progression. Our findings suggest that tumor antigens are handled as self antigens, and peripheral tolerance is associated with in situ antigen overexpression and cancer progression. Our data also support a relevant role for DC-based vaccines in controlling the induction of peripheral tolerance to tumor antigens.

Original languageEnglish
Pages (from-to)66-75
Number of pages10
JournalEuropean Journal of Immunology
Volume35
Issue number1
DOIs
Publication statusPublished - Jan 2005

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Dendritic Cells
Prostate
Immunization
Prostatic Neoplasms
Viral Tumor Antigens
Peripheral Tolerance
T-Lymphocytes
Transgenic Mice
Adenocarcinoma
Cytotoxic T-Lymphocytes
Neoplasm Antigens
Neoplasms
Polyomavirus Transforming Antigens
Intradermal Injections
Autoantigens
Disease Progression
Histology
Vaccination
Vaccines
Epithelium

Keywords

  • Cancer immunity
  • CTL
  • Dendritic cells
  • Rodent
  • Tolerance/anergy

ASJC Scopus subject areas

  • Immunology

Cite this

Peripheral T cell tolerance occurs early during spontaneous prostate cancer development and can be rescued by dendritic cell immunization. / Degl'Innocenti, Elena; Grioni, Matteo; Boni, Andrea; Camporeale, Annalisa; Bertilaccio, Maria T S; Freschi, Massimo; Monno, Antonella; Arcelloni, Cinzia; Greenberg, Norman M.; Bellone, Matteo.

In: European Journal of Immunology, Vol. 35, No. 1, 01.2005, p. 66-75.

Research output: Contribution to journalArticle

Degl'Innocenti, E, Grioni, M, Boni, A, Camporeale, A, Bertilaccio, MTS, Freschi, M, Monno, A, Arcelloni, C, Greenberg, NM & Bellone, M 2005, 'Peripheral T cell tolerance occurs early during spontaneous prostate cancer development and can be rescued by dendritic cell immunization', European Journal of Immunology, vol. 35, no. 1, pp. 66-75. https://doi.org/10.1002/eji.200425531
Degl'Innocenti, Elena ; Grioni, Matteo ; Boni, Andrea ; Camporeale, Annalisa ; Bertilaccio, Maria T S ; Freschi, Massimo ; Monno, Antonella ; Arcelloni, Cinzia ; Greenberg, Norman M. ; Bellone, Matteo. / Peripheral T cell tolerance occurs early during spontaneous prostate cancer development and can be rescued by dendritic cell immunization. In: European Journal of Immunology. 2005 ; Vol. 35, No. 1. pp. 66-75.
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