Peritoneal expression of matrilysin helps identify early post-operative recurrence of colorectal cancer

Giuseppe S. Sica, Cristina Fiorani, Carmine Stolfi, Giovanni Monteleone, Eleonora Candi, Ivano Amelio, Valeria Catani, Simone Sibio, Andrea Divizia, Giorgia Tema, Edoardo Iaculli, Achille L. Gaspari

Research output: Contribution to journalArticle

Abstract

Recurrence of colorectal cancer (CRC) following a potentially curative resection is a challenging clinical problem. Matrix metalloproteinase-7 (MMP-7) is overexpressed by CRC cells and supposed to play a major role in CRC cell diffusion and metastasis. MMP-7 RNA expression was assessed by real-time PCR using specific primers in peritoneal washing fluid obtained during surgical procedure. After surgery, patients underwent a regular follow up for assessing recurrence. transcripts for MMP-7 were detected in 31/57 samples (54%). Patients were followed-up (range 20-48 months) for recurrence prevention. Recurrence was diagnosed in 6 out of 55 patients (11%) and two patients eventually died because of this. Notably, all the six patients who had relapsed were positive for MMP-7. Sensitivity and specificity of the test were 100% and 49% respectively. Data from patients have also been corroborated by computational approaches. Public available coloncarcinoma datasets have been employed to confirm MMP7 clinical impact on the disease. Interestingly, MMP-7 expression appeared correlated to Tgfb-1, and correlation of the two factors represented a poor prognostic factor. This study proposes positivity of MMP-7 in peritoneal cavity as a novel biomarker for predicting disease recurrence in patients with CRC.

Original languageEnglish
Pages (from-to)13402-13415
Number of pages14
JournalOncotarget
Volume6
Issue number15
Publication statusPublished - 2015

    Fingerprint

ASJC Scopus subject areas

  • Oncology

Cite this

Sica, G. S., Fiorani, C., Stolfi, C., Monteleone, G., Candi, E., Amelio, I., Catani, V., Sibio, S., Divizia, A., Tema, G., Iaculli, E., & Gaspari, A. L. (2015). Peritoneal expression of matrilysin helps identify early post-operative recurrence of colorectal cancer. Oncotarget, 6(15), 13402-13415.