Peritoneal T cell responses can be polarized toward Th1 or Th2 in children on chronic peritoneal dialysis

Sabrina Chiesa, Giacomo Vigo, Francesca Cappa, Ignazia Prigione, Vito Pistoia, Enrico Verrina, Francesco Perfumo, Giancarlo Barbano

Research output: Contribution to journalArticlepeer-review

Abstract

Peritoneal T cell responses can be polarized toward Th1 or Th2 in children on chronic peritoneal dialysis. Previous studies on the peritoneal immune system described the presence of activated T lymphocytes in peritoneal effluents from subjects on chronic peritoneal dialysis (CPD). Since Th1/Th2 polarized response can influence the outcome of specific infectious diseases, we investigated if activated Th1/Th2 cells can be detected in peritoneal effluents during peritoneal dialysis, in order to better understand the role of T cells in the mechanisms of peritoneal defense. We have studied 8 children (4 males, 4 females, mean age 5.8 ± 5.7 years, range 0.3-13.4) on CPD. Peritoneal cells have been isolated from peritoneal effluents by centrifugation. Immunofluorescent staining of intracellular cytokines for flow cytometric analysis was used to detect the percentage of T cells producing either IFN-γ (Th1) or IL-4 (Th2). In the initial study 3 months after CPD initiation, high percentages of IFN-γ positive peritoneal T cells (38% and 63%) were detected in two subjects; this finding is consistent with a Th1 polarization of peritoneal T cells. In another subject, high percentages of IL-4 positive T cells (31%) were detected, suggesting a Th2 polarization of peritoneal T cell response. Small amounts of either Th1 or Th2 T cells (2-4%) were also detected in the other subjects. At the 1 year follow-up, Th1 polarization persisted in one subject (18% IFN-γ positive peritoneal T cells), in another a shift from Th1 to Th2 was observed, and in the other subject a down regulation of both T cell subsets occurred. The finding that a predominance of T cells producing either IFN-γ or IL-4 was found in 3 out of 8 children strongly suggests that peritoneal T cell responses can be polarized toward Th1 or Th2. The decrease of Th1 and/or Th2 polarized T cells in the peritoneum of 4 out of 6 subjects (after 1 year) suggests that CPD can play an immunosuppressive role on T cell peritoneal responses. Further studies are needed in order to define whether different T helper activation patterns are associated with a higher risk of peritoneal infection or of peritoneal damage.

Original languageEnglish
Pages (from-to)750-753
Number of pages4
JournalArtificial Organs
Volume28
Issue number8
DOIs
Publication statusPublished - Aug 2004

Keywords

  • Children
  • IFN-γ
  • IL-4
  • Peritoneal dialysis
  • T lymphocytes
  • Th1
  • Th2

ASJC Scopus subject areas

  • Biophysics

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