Perivascular carotid collar placement induces neointima formation and outward arterial remodeling in mice independent of apolipoprotein E deficiency or Western-type diet feeding

Roberta Baetta, Francesca Silva, Carmen Comparato, Marta Uzzo, Ivano Eberini, Stefano Bellosta, Elena Donetti, Alberto Corsini

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We investigated the influence of apolipoprotein E deficiency and Western-type diet feeding on the development and composition of neointimal lesions induced by periadventitial carotid placement of a non-occlusive collar in mice. ApoE-/- and wild-type mice were fed a Western-type diet or chow diet for 4 weeks before collar surgery. Diets were continued after collar placement for 6 or 12 weeks. Compared to sham-operated arteries, collared carotids showed significant neointima formation, lumen loss, and outward remodeling in both apoE-/- and wild-type mice. These changes were not affected by either the genotype or the diet. Conversely, significant differences in neointima composition were detected between the two genotypes, with apoE-/- mice showing greater lipid deposition and lower SMC accumulation compared to wild-type mice, independent of the dietetic regimen. Altogether, the results of the present study indicate that although lesion composition may be influenced by genotype, neointima formation and arterial remodeling in the murine perivascular carotid collar model occur independent of the exposure to atherogenic diet or the presence of a sensitized genotype such as apoE-/-. The murine perivascular carotid collar model would thus be suitable for investigating neointima formation, arterial remodeling, and their potential pharmacological modulation in the setting of different genetic and dietary conditions.

Original languageEnglish
JournalAtherosclerosis
Volume195
Issue number1
DOIs
Publication statusPublished - Nov 2007

Fingerprint

Neointima
Apolipoproteins E
Genotype
Diet
Atherogenic Diet
Dietetics
Carotid Arteries
Western Diet
Pharmacology
Lipids

Keywords

  • Animal models
  • Atherosclerosis
  • Carotid arteries
  • Neutrophils

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Perivascular carotid collar placement induces neointima formation and outward arterial remodeling in mice independent of apolipoprotein E deficiency or Western-type diet feeding. / Baetta, Roberta; Silva, Francesca; Comparato, Carmen; Uzzo, Marta; Eberini, Ivano; Bellosta, Stefano; Donetti, Elena; Corsini, Alberto.

In: Atherosclerosis, Vol. 195, No. 1, 11.2007.

Research output: Contribution to journalArticle

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AU - Baetta, Roberta

AU - Silva, Francesca

AU - Comparato, Carmen

AU - Uzzo, Marta

AU - Eberini, Ivano

AU - Bellosta, Stefano

AU - Donetti, Elena

AU - Corsini, Alberto

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