Perlecan protein core interacts with extracellular matrix protein 1 (ECM1), a glycoprotein involved in bone formation and angiogenesis

Maurizio Mongiat, Jian Fu, Rachel Oldershaw, Robert Greenhalgh, Allen M. Gown, Renato V. Iozzo

Research output: Contribution to journalArticle

Abstract

The goal of this study was to discover novel partners for perlecan, a major heparan sulfate proteoglycan of basement membranes, and to examine new interactions through which perlecan may influence cell behavior. We employed the yeast two-hybrid system and used perlecan domain V as bait to screen a human keratinocyte cDNA library. Among the strongest interacting clones, we isolated a ∼1.6-kb cDNA insert that encoded extracellular matrix protein 1 (ECM1), a secreted glycoprotein involved in bone formation and angiogenesis. The sequencing of the clone revealed the existence of a novel splice variant that we name ECM1c. The interaction was validated by co-immunoprecipitation studies, using both cell-free systems and mammalian cells, and the specific binding site within each molecule was identified employing various deletion mutants. The C terminus of ECM1 interacted specifically with the epidermal growth factor-like modules flanking the LG2 subdomain of perlecan domain V. Perlecan and ECM1 were also co-expressed by a variety of normal and transformed cells, and immunohistochemical studies showed a partial expression overlap, particularly around dermal blood vessels and adnexal epithelia. ECM1 has been shown to regulate endochondral bone formation, stimulate the proliferation of endothelial cells, and induce angiogenesis. Similarly, perlecan plays an important role in chondrogenesis and skeletal development, as well as harboring pro- and anti-angiogenic activities. Thus, a physiological interaction could also occur in vivo during development and in pathological events, including tissue remodeling and tumor progression.

Original languageEnglish
Pages (from-to)17491-17499
Number of pages9
JournalJournal of Biological Chemistry
Volume278
Issue number19
DOIs
Publication statusPublished - May 9 2003

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Extracellular Matrix Proteins
Osteogenesis
Glycoproteins
Bone
Proteins
Clone Cells
Chondrogenesis
Heparan Sulfate Proteoglycans
Two-Hybrid System Techniques
Cell-Free System
Endothelial cells
Blood vessels
Hybrid systems
Gene Library
Keratinocytes
Immunoprecipitation
Epidermal Growth Factor
Basement Membrane
Yeast
Names

ASJC Scopus subject areas

  • Biochemistry

Cite this

Perlecan protein core interacts with extracellular matrix protein 1 (ECM1), a glycoprotein involved in bone formation and angiogenesis. / Mongiat, Maurizio; Fu, Jian; Oldershaw, Rachel; Greenhalgh, Robert; Gown, Allen M.; Iozzo, Renato V.

In: Journal of Biological Chemistry, Vol. 278, No. 19, 09.05.2003, p. 17491-17499.

Research output: Contribution to journalArticle

Mongiat, Maurizio ; Fu, Jian ; Oldershaw, Rachel ; Greenhalgh, Robert ; Gown, Allen M. ; Iozzo, Renato V. / Perlecan protein core interacts with extracellular matrix protein 1 (ECM1), a glycoprotein involved in bone formation and angiogenesis. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 19. pp. 17491-17499.
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