Peroxidated squalene induces the production of inflammatory mediators in HaCaT keratinocytes: A possible role in acne vulgaris

Monica Ottaviani, Theodosis Alestas, Enrica Flori, Arianna Mastrofrancesco, Christos C. Zouboulis, Mauro Picardo

Research output: Contribution to journalArticle

Abstract

In order to investigate whether products derived from the oxidation of sebum can be responsible for the induction of inflammatory processes, HaCaT keratinocytes were treated with peroxidated squalene. NF-κB activation, secretion, and expression of IL-6, as well as peroxisome proliferator-activated receptor alpha (PPARα) mRNA and protein levels, were measured at the end of the treatment and after 24 and 48 hours of recovery. Squalene peroxidation products were administered in amounts able to elicit significant hyperproliferation and to induce lipoxygenase (LOX) activity. The results showed an early activation of NF-κB followed by an increase in PPARα mRNA and protein levels. Moreover, squalene peroxides induced an initial upregulation of IL-6 production and secretion that was counteracted by PPARα activation, as suggested by the subsequent decrease of NF-κB nuclear translocation and IL-6 levels. Inflammatory processes play an important role in the development of acne vulgaris. In combination with our own previous findings, which indicated an association between LOX stimulation and increased percentage of proinflammatory lipids in acne as well as a correlation between increased cytokine levels in the infundibulum, pilosebaceous duct hyperkeratinization, and augmented sebogenesis, the present data further support the involvement of lipid peroxides, in particular squalene peroxides, in establishing an inflammatory process in acne.

Original languageEnglish
Pages (from-to)2430-2437
Number of pages8
JournalJournal of Investigative Dermatology
Volume126
Issue number11
DOIs
Publication statusPublished - Nov 15 2006

ASJC Scopus subject areas

  • Dermatology

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