Peroxidative damage of the erythrocyte membrane in children with nephrotic syndrome

Fabrizio Ginevri, Gian Marco Ghiggeri, Giovanni Candiano, Roberta Oleggini, Roberta Bertelli, Maria Teresa Piccardo, Francesco Perfumo, Rosanna Gusmano

Research output: Contribution to journalArticlepeer-review

Abstract

The structural composition of erythrocyte ghosts was analysed in children affected by steroid-responsive (SRNS) and unresponsive nephrotic syndrome (SUNS). No variation of either intrinsic or extrinsic ghost proteins was found by discontinuous SDS-electrophoresis associated with a very sensitive double staining technique. By contrast, the composition of inner-layer phospholipids - phosphatidyl ethanolamine (PE) and phosphatidyl serine (PS) - was altered in SRNS with minor changes also involving phosphatidic acid, phosphatidyl inositol and lysophosphatidyl choline. Signs of peroxidative damage were present in both SRNS and SUNS ghosts and inside the cells; these included high levels of fluorescent amino-iminopropene derivates of PE and PS, increased intraerythrocytic amounts of malonyldialdehyde and decreased levels of reduced glutathione. Taken together these results support the concept that in SRNS and SUNS erythrocytes are target cells for peroxidative damage. In SRNS peroxidation of membrane lipids results in a marked alteration of the phospholipid composition of erythrocyte ghosts.

Original languageEnglish
Pages (from-to)25-32
Number of pages8
JournalPediatric Nephrology
Volume3
Issue number1
DOIs
Publication statusPublished - Mar 1989

Keywords

  • Amino
  • Damage, peroxidative
  • Erythrocyte
  • Glutathione
  • iminopropene compounds
  • Malonyldialdehyde
  • Nephrotic syndrome
  • Phospholipid

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Nephrology

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