TY - JOUR
T1 - Peroxiredoxin 2 nuclear levels are regulated by circadian clock synchronization in human keratinocytes
AU - Avitabile, Daniele
AU - Ranieri, Danilo
AU - Nicolussi, Arianna
AU - D'Inzeo, Sonia
AU - Capriotti, Anna Laura
AU - Genovese, Licia
AU - Proietti, Sara
AU - Cucina, Alessandra
AU - Coppa, Anna
AU - Samperi, Roberto
AU - Bizzarri, Mariano
AU - Laganà, Aldo
AU - Torrisi, Maria Rosaria
PY - 2014
Y1 - 2014
N2 - Circadian rhythms are highly conserved time tracking systems regulating important biological processes at both systemic and cellular levels. The present study was aimed to identify proteins and biological functions circadian regulated in human keratinocytes. HaCaT keratinocytes were entrained by temperature cycles, and a proteomic study was performed on cell fractions isolated under free running conditions at constant temperature. Bioinformatics analysis revealed that molecular clock entrainment was associated with changes in molecular components regulating cell proliferation, energy metabolism, transcription, translation and redox balance. Nuclear levels of the antioxidant enzyme Peroxiredoxin 2 (PRDX2) were found to oscillate rhythmically over two entire 24 h long cycles. Donwregulation of PRDX2 resulted in upregulation of the mitochondrion-specific Peroxiredoxin 3 (PRDX3), all other members of the Peroxiredoxin family remained unaltered. Furthermore, PRDX2 knockdown increased intracellular levels of reactive oxygen species (ROS) and impaired cell cycle progression and proliferation. HaCaT cells transduced with a scramble shRNA were used as control. Our work is the first to show that nuclear levels of PRDX2 display circadian oscillation participating in the regulation of human keratinocytes redox balance.
AB - Circadian rhythms are highly conserved time tracking systems regulating important biological processes at both systemic and cellular levels. The present study was aimed to identify proteins and biological functions circadian regulated in human keratinocytes. HaCaT keratinocytes were entrained by temperature cycles, and a proteomic study was performed on cell fractions isolated under free running conditions at constant temperature. Bioinformatics analysis revealed that molecular clock entrainment was associated with changes in molecular components regulating cell proliferation, energy metabolism, transcription, translation and redox balance. Nuclear levels of the antioxidant enzyme Peroxiredoxin 2 (PRDX2) were found to oscillate rhythmically over two entire 24 h long cycles. Donwregulation of PRDX2 resulted in upregulation of the mitochondrion-specific Peroxiredoxin 3 (PRDX3), all other members of the Peroxiredoxin family remained unaltered. Furthermore, PRDX2 knockdown increased intracellular levels of reactive oxygen species (ROS) and impaired cell cycle progression and proliferation. HaCaT cells transduced with a scramble shRNA were used as control. Our work is the first to show that nuclear levels of PRDX2 display circadian oscillation participating in the regulation of human keratinocytes redox balance.
KW - Circadian rhythm
KW - HaCaT keratinocytes
KW - Metabolic clock
KW - Nano-LC-MS/MS
KW - Peroxiredoxin 2
KW - Proteomic analysis
UR - http://www.scopus.com/inward/record.url?scp=84901262708&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901262708&partnerID=8YFLogxK
U2 - 10.1016/j.biocel.2014.04.024
DO - 10.1016/j.biocel.2014.04.024
M3 - Article
C2 - 24814289
AN - SCOPUS:84901262708
VL - 53
SP - 24
EP - 34
JO - International Journal of Biochemistry and Cell Biology
JF - International Journal of Biochemistry and Cell Biology
SN - 1357-2725
ER -