Peroxisome proliferator-activated receptor-α and -γ mRNA levels are reduced in chronic hepatitis C with steatosis and genotype 3 infection

A. De Gottardi, V. Pazienza, P. Pugnale, F. Bruttin, L. Rubbia-Brandt, C. E. Juge-Aubry, C. A. Meier, A. Hadengue, F. Negro

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Steatosis in chronic hepatitis C is associated with inflammation and accelerated fibrogenesis. Aim: To assess the contribution of peroxisome proliferator-activated receptor-α and -γ to the pathogenesis of hepatitis C virus associated steatosis is unknown. Methods: We measured peroxisome proliferator-activated receptor (PPAR)-α and -γ mRNA by quantitative polymerase chain reaction in liver biopsies of 35 genotype 1 and 22 genotype 3 infected patients and in Huh7 cells expressing hepatitis C virus 1b or 3a core protein. Results: PPAR-α mRNA was significantly reduced in livers of patients with genotype 3 compared with genotype 1. Steatosis was associated to a decreased expression of PPAR-α in genotype 1, but not in genotype 3. PPAR-γ expression was significantly lower in genotype 3 compared with genotype 1 and steatosis was associated to decreased levels of PPAR-γ, but only in genotype 1. There was no significant relationship between PPARs mRNA levels and liver activity or fibrosis. Expression of the hepatitis C virus 3a core protein was associated with an increase in triglyceride accumulation and with a significant reduction of PPAR-γ mRNA compared with hepatitis C virus 1b. Conclusions: The presence of steatosis and hepatitis C virus genotype 3 are both associated with a significant down-regulation of PPARs. These receptors, and also additional factors, seem to play a role in the pathogenesis of hepatitis C virus-associated steatosis.

Original languageEnglish
Pages (from-to)107-114
Number of pages8
JournalAlimentary Pharmacology and Therapeutics
Volume23
Issue number1
DOIs
Publication statusPublished - Jan 2006

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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