Peroxynitrite decomposition catalyst prevents apoptotic cell death in a human astrocytoma cell line incubated with supernatants of HIV-infected macrophages

Carolina Muscoli, Daniela Salvemini, Donatella Paolino, Michelangelo Iannone, Ernesto Palma, Antonio Cufari, Domenicantonio Rotiroti, Carlo Federico Perno, Stefano Aquaro, Vincenzo Mollace

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Abstract

Background: Oxidative stress has shown to contribute in the mechanisms underlying apoptotic cell death occuring in AIDS-dementia complex. Here we investigated the role of peroxynitrite in apoptosis occurring in astroglial cells incubated with supernatants of HIV-infected human primary macrophages (M/M). Results: Flow cytometric analysis (FACS) of human cultured astrocytes shortly incubated with HIV-1-infected M/M supernatants showed apoptotic cell death, an effect accompanied by pronounced staining for nitrotyrosine (footprint of peroxynitrite) and by abnormal formation of malondialdehyde (MDA). Pretreatment of astrocytes with the peroxynitrite decomposition catalyst FeTMPS antagonized HIV-related astrocytic apoptosis, MDA formation and nitrotyrosine staining. Conclusions: Taken together, our results suggest that inibition of peroxynitrite leads to protection against peroxidative stress accompanying HIV-related apoptosis of astrocytes. Overall results support the role of peroxynitrite in HIV-related programmed death of astrocytes and suggest the use of peroxynitrite decomposition catalyst to counteract HIV-1-related neurological disorders.

Original languageEnglish
Article number13
JournalBMC Neuroscience
Volume3
DOIs
Publication statusPublished - Sep 16 2002

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Keywords

  • AIDS dementia complex
  • Astroglial cells
  • FeTPMS
  • Peroxynitrite
  • Therapy

ASJC Scopus subject areas

  • Medicine(all)
  • Clinical Neurology
  • Neuroscience(all)

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