Abstract
Globins protect from the oxidative and nitrosative cell damage. Here, kinetics of peroxynitrite scavenging by ferryl sperm whale myoglobin (Mb{single bond}Fe(IV){double bond, long}O) and human hemoglobin (Hb{single bond}Fe(IV){double bond, long}O), between pH 5.8 and 8.3 at 20.0 °C, are reported. In the absence of CO2, values of the second-order rate constant for peroxynitrite scavenging by Mb{single bond}Fe(IV){double bond, long}O and Hb{single bond}Fe(IV){double bond, long}O (i.e., for Mb{single bond}Fe(III) and Hb{single bond}Fe(III) formation; kon) are 4.6 × 104 M-1 s-1 and 3.3 × 104 M-1 s-1, respectively, at pH 7.1. Values of kon increase on decreasing pH with pKa values of 6.9 and 6.7, this suggests that the ONOOH species reacts preferentially with Mb{single bond}Fe(IV){double bond, long}O and Hb{single bond}Fe(IV){double bond, long}O. In the presence of CO2 (=1.2 × 10-3 M), values of kon for peroxynitrite scavenging by Mb{single bond}Fe(IV){double bond, long}O and Hb{single bond}Fe(IV){double bond, long}O are essentially pH-independent, the average kon values are 7.1 × 104 M-1 s-1 and 1.2 × 105 M-1 s-1, respectively. As a whole, Mb{single bond}Fe(IV){double bond, long}O and Hb{single bond}Fe(IV){double bond, long}O, obtained by treatment with H2O2, undertake within the same cycle H2O2 and peroxynitrite detoxification.
| Original language | English |
|---|---|
| Pages (from-to) | 27-31 |
| Number of pages | 5 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 390 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Dec 4 2009 |
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Keywords
- Effect of CO
- Ferric hemoglobin formation
- Ferric myoglobin formation
- Ferryl hemoglobin reduction
- Ferryl myoglobin reduction
- Kinetics
- Peroxynitrite scavenging
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Cell Biology
- Molecular Biology
Cite this
Peroxynitrite scavenging by ferryl sperm whale myoglobin and human hemoglobin. / Ascenzi, Paolo; Marinis, Elisabetta De; Masi, Alessandra di; Ciaccio, Chiara; Coletta, Massimo.
In: Biochemical and Biophysical Research Communications, Vol. 390, No. 1, 04.12.2009, p. 27-31.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Peroxynitrite scavenging by ferryl sperm whale myoglobin and human hemoglobin
AU - Ascenzi, Paolo
AU - Marinis, Elisabetta De
AU - Masi, Alessandra di
AU - Ciaccio, Chiara
AU - Coletta, Massimo
PY - 2009/12/4
Y1 - 2009/12/4
N2 - Globins protect from the oxidative and nitrosative cell damage. Here, kinetics of peroxynitrite scavenging by ferryl sperm whale myoglobin (Mb{single bond}Fe(IV){double bond, long}O) and human hemoglobin (Hb{single bond}Fe(IV){double bond, long}O), between pH 5.8 and 8.3 at 20.0 °C, are reported. In the absence of CO2, values of the second-order rate constant for peroxynitrite scavenging by Mb{single bond}Fe(IV){double bond, long}O and Hb{single bond}Fe(IV){double bond, long}O (i.e., for Mb{single bond}Fe(III) and Hb{single bond}Fe(III) formation; kon) are 4.6 × 104 M-1 s-1 and 3.3 × 104 M-1 s-1, respectively, at pH 7.1. Values of kon increase on decreasing pH with pKa values of 6.9 and 6.7, this suggests that the ONOOH species reacts preferentially with Mb{single bond}Fe(IV){double bond, long}O and Hb{single bond}Fe(IV){double bond, long}O. In the presence of CO2 (=1.2 × 10-3 M), values of kon for peroxynitrite scavenging by Mb{single bond}Fe(IV){double bond, long}O and Hb{single bond}Fe(IV){double bond, long}O are essentially pH-independent, the average kon values are 7.1 × 104 M-1 s-1 and 1.2 × 105 M-1 s-1, respectively. As a whole, Mb{single bond}Fe(IV){double bond, long}O and Hb{single bond}Fe(IV){double bond, long}O, obtained by treatment with H2O2, undertake within the same cycle H2O2 and peroxynitrite detoxification.
AB - Globins protect from the oxidative and nitrosative cell damage. Here, kinetics of peroxynitrite scavenging by ferryl sperm whale myoglobin (Mb{single bond}Fe(IV){double bond, long}O) and human hemoglobin (Hb{single bond}Fe(IV){double bond, long}O), between pH 5.8 and 8.3 at 20.0 °C, are reported. In the absence of CO2, values of the second-order rate constant for peroxynitrite scavenging by Mb{single bond}Fe(IV){double bond, long}O and Hb{single bond}Fe(IV){double bond, long}O (i.e., for Mb{single bond}Fe(III) and Hb{single bond}Fe(III) formation; kon) are 4.6 × 104 M-1 s-1 and 3.3 × 104 M-1 s-1, respectively, at pH 7.1. Values of kon increase on decreasing pH with pKa values of 6.9 and 6.7, this suggests that the ONOOH species reacts preferentially with Mb{single bond}Fe(IV){double bond, long}O and Hb{single bond}Fe(IV){double bond, long}O. In the presence of CO2 (=1.2 × 10-3 M), values of kon for peroxynitrite scavenging by Mb{single bond}Fe(IV){double bond, long}O and Hb{single bond}Fe(IV){double bond, long}O are essentially pH-independent, the average kon values are 7.1 × 104 M-1 s-1 and 1.2 × 105 M-1 s-1, respectively. As a whole, Mb{single bond}Fe(IV){double bond, long}O and Hb{single bond}Fe(IV){double bond, long}O, obtained by treatment with H2O2, undertake within the same cycle H2O2 and peroxynitrite detoxification.
KW - Effect of CO
KW - Ferric hemoglobin formation
KW - Ferric myoglobin formation
KW - Ferryl hemoglobin reduction
KW - Ferryl myoglobin reduction
KW - Kinetics
KW - Peroxynitrite scavenging
UR - http://www.scopus.com/inward/record.url?scp=70349975706&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70349975706&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2009.09.050
DO - 10.1016/j.bbrc.2009.09.050
M3 - Article
C2 - 19766099
AN - SCOPUS:70349975706
VL - 390
SP - 27
EP - 31
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -