Persistence of non clonal hematopoietic progenitor cells in blastic phase chronic myelogenous leukemia (CML)

G. Martinelli, R. M. Lemoli, P. Farabegoli, A. Zaccaria, N. Testoni, M. Buzzi, M. Fogli, G. Visani, P. Tosi, M. R. Motta, S. Rizzi, A. Fortuna, M. Amabile, S. Tura

Research output: Contribution to journalArticle

Abstract

Normal and clonal hematopoietic progenitor cells have been demonstrated to coexist in chronic-phase chronic myelogenous leukemia (CML), but few data are available on the presence of non neoplastic hematopoiesis during the blastic transformation phase. We used reverse transcription-polymerase chain reaction (RT-PCR) to investigate expression of the BCR/ABL transcript of individual hematopoietic progenitors from a CML patient in blastic phase. We demonstrate that non clonal hematopoiesis is induced to re-emerge by conventional chemotherapy containing fludarabine®. In addition, we confirm that some pluripotent CD34+/CD33-/DR- cells circulating in the peripheral blood are not clonal. Our data provide an encouraging basis for further studies of in vitro purification of normal hematopoietic stem cells in advanced stage CML and of their use in the context of autologous bone marrow transplantation.

Original languageEnglish
Pages (from-to)445-447
Number of pages3
JournalHaematologica
Volume79
Issue number5
Publication statusPublished - 1994

Keywords

  • BCR-ABL mRNA
  • blastic phase
  • chronic myelogenous leukemia
  • hematopoiesis
  • PCR
  • stem cell

ASJC Scopus subject areas

  • Hematology

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  • Cite this

    Martinelli, G., Lemoli, R. M., Farabegoli, P., Zaccaria, A., Testoni, N., Buzzi, M., Fogli, M., Visani, G., Tosi, P., Motta, M. R., Rizzi, S., Fortuna, A., Amabile, M., & Tura, S. (1994). Persistence of non clonal hematopoietic progenitor cells in blastic phase chronic myelogenous leukemia (CML). Haematologica, 79(5), 445-447.