TY - JOUR
T1 - Persistence of T-cell immune response induced by two acellular pertussis vaccines in children five years after primary vaccination
AU - Palazzo, Raffaella
AU - Carollo, Maria
AU - Bianco, Manuela
AU - Fedele, Giorgio
AU - Schiavoni, Ilaria
AU - Pandolfi, Elisabetta
AU - Villani, Alberto
AU - Tozzi, Alberto E.
AU - Mascart, Françoise
AU - Ausiello, Clara M.
PY - 2016/1/1
Y1 - 2016/1/1
N2 - The resurgence of pertussis suggests the need for greater efforts to understand the long-lasting protective responses induced by vaccination. In this paper we dissect the persistence of T memory responses induced by primary vaccination with two different acellular pertussis (aP) vaccines, hexavalent Hexavac vaccine (Hexavac) (Sanofi Pasteur MSD) and Infanrix hexa (Infanrix) (Glaxo-SmithKline Biologicals). We evaluated magnitude and duration of T-cell responses to pertussis toxin (PT) by measuring T-cell proliferation, cytokines (IL-2 and IFNγ) production and memory subsets in two groups of children 5 years after primary vaccination. Some of the enrolled children received only primary vaccination, while others had the pre-school boost dose. Positive T-cell responses to PT were detected in 36% of children. Percentage of responsive children, T-cell proliferation and CD4IL-2+ cells were significantly higher in the children primed with Hexavac than in those who received Infanrix vaccine. No major effects of the boost on PT-specific proliferation were observed. Overall, our data documented a persistence of T-cell memory against PT in a minor fraction of children 5 years after primary vaccination. The different responses induced by Hexavac and Infanrix vaccine could rely on differences in PT inactivation process or excipients/adjuvants formulations.
AB - The resurgence of pertussis suggests the need for greater efforts to understand the long-lasting protective responses induced by vaccination. In this paper we dissect the persistence of T memory responses induced by primary vaccination with two different acellular pertussis (aP) vaccines, hexavalent Hexavac vaccine (Hexavac) (Sanofi Pasteur MSD) and Infanrix hexa (Infanrix) (Glaxo-SmithKline Biologicals). We evaluated magnitude and duration of T-cell responses to pertussis toxin (PT) by measuring T-cell proliferation, cytokines (IL-2 and IFNγ) production and memory subsets in two groups of children 5 years after primary vaccination. Some of the enrolled children received only primary vaccination, while others had the pre-school boost dose. Positive T-cell responses to PT were detected in 36% of children. Percentage of responsive children, T-cell proliferation and CD4IL-2+ cells were significantly higher in the children primed with Hexavac than in those who received Infanrix vaccine. No major effects of the boost on PT-specific proliferation were observed. Overall, our data documented a persistence of T-cell memory against PT in a minor fraction of children 5 years after primary vaccination. The different responses induced by Hexavac and Infanrix vaccine could rely on differences in PT inactivation process or excipients/adjuvants formulations.
KW - Acellular pertussis vaccine
KW - Cytokines
KW - Memory T-cell subsets
KW - Pertussis resurgence
KW - T-cell responses; Proliferation
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M3 - Article
AN - SCOPUS:84959421551
VL - 39
SP - 45
EP - 57
JO - New Microbiologica
JF - New Microbiologica
SN - 1121-7138
IS - 1
ER -