Abstract
In the last two decades, several phage display-selected monoclonal antibodies (mAbs) have been described in the literature and a few of them have managed to reach the clinics. Among these, the anti-respiratory syncytial virus (RSV) Palivizumab, a phage-display optimized mAb, is the only marketed mAb directed against microbial pathogens. Palivizumab is a clear example of the importance of choosing the most appropriate strategy when selecting or optimizing an anti-infectious mAb. From this perspective, the extreme versatility of phage-display technology makes it a useful tool when setting up different strategies for the selection of mAbs directed against human pathogens, especially when their possible clinical use is considered. In this paper, we review the principal phage display strategies used to select anti-infectious mAbs, with particular attention focused on those used against hypervariable pathogens, such as HCV and influenza viruses.
| Original language | English |
|---|---|
| Pages (from-to) | 8273-8292 |
| Number of pages | 20 |
| Journal | International Journal of Molecular Sciences |
| Volume | 13 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 2012 |
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Keywords
- Biopanning
- Hypervariable pathogens
- Library construction
- Monoclonal antibodies
- Phage display
ASJC Scopus subject areas
- Physical and Theoretical Chemistry
- Organic Chemistry
- Spectroscopy
- Inorganic Chemistry
- Catalysis
- Molecular Biology
- Computer Science Applications
- Medicine(all)
Cite this
Phage display-based strategies for cloning and optimization of monoclonal antibodies directed against human pathogens. / Clementi, Nicola; Mancini, Nicasio; Solforosi, Laura; Castelli, Matteo; Clementi, Massimo; Burioni, Roberto.
In: International Journal of Molecular Sciences, Vol. 13, No. 7, 2012, p. 8273-8292.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Phage display-based strategies for cloning and optimization of monoclonal antibodies directed against human pathogens
AU - Clementi, Nicola
AU - Mancini, Nicasio
AU - Solforosi, Laura
AU - Castelli, Matteo
AU - Clementi, Massimo
AU - Burioni, Roberto
PY - 2012
Y1 - 2012
N2 - In the last two decades, several phage display-selected monoclonal antibodies (mAbs) have been described in the literature and a few of them have managed to reach the clinics. Among these, the anti-respiratory syncytial virus (RSV) Palivizumab, a phage-display optimized mAb, is the only marketed mAb directed against microbial pathogens. Palivizumab is a clear example of the importance of choosing the most appropriate strategy when selecting or optimizing an anti-infectious mAb. From this perspective, the extreme versatility of phage-display technology makes it a useful tool when setting up different strategies for the selection of mAbs directed against human pathogens, especially when their possible clinical use is considered. In this paper, we review the principal phage display strategies used to select anti-infectious mAbs, with particular attention focused on those used against hypervariable pathogens, such as HCV and influenza viruses.
AB - In the last two decades, several phage display-selected monoclonal antibodies (mAbs) have been described in the literature and a few of them have managed to reach the clinics. Among these, the anti-respiratory syncytial virus (RSV) Palivizumab, a phage-display optimized mAb, is the only marketed mAb directed against microbial pathogens. Palivizumab is a clear example of the importance of choosing the most appropriate strategy when selecting or optimizing an anti-infectious mAb. From this perspective, the extreme versatility of phage-display technology makes it a useful tool when setting up different strategies for the selection of mAbs directed against human pathogens, especially when their possible clinical use is considered. In this paper, we review the principal phage display strategies used to select anti-infectious mAbs, with particular attention focused on those used against hypervariable pathogens, such as HCV and influenza viruses.
KW - Biopanning
KW - Hypervariable pathogens
KW - Library construction
KW - Monoclonal antibodies
KW - Phage display
UR - http://www.scopus.com/inward/record.url?scp=84864350649&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84864350649&partnerID=8YFLogxK
U2 - 10.3390/ijms13078273
DO - 10.3390/ijms13078273
M3 - Article
C2 - 22942702
AN - SCOPUS:84864350649
VL - 13
SP - 8273
EP - 8292
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1661-6596
IS - 7
ER -