Phage display-based strategies for cloning and optimization of monoclonal antibodies directed against human pathogens

Nicola Clementi, Nicasio Mancini, Laura Solforosi, Matteo Castelli, Massimo Clementi, Roberto Burioni

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

In the last two decades, several phage display-selected monoclonal antibodies (mAbs) have been described in the literature and a few of them have managed to reach the clinics. Among these, the anti-respiratory syncytial virus (RSV) Palivizumab, a phage-display optimized mAb, is the only marketed mAb directed against microbial pathogens. Palivizumab is a clear example of the importance of choosing the most appropriate strategy when selecting or optimizing an anti-infectious mAb. From this perspective, the extreme versatility of phage-display technology makes it a useful tool when setting up different strategies for the selection of mAbs directed against human pathogens, especially when their possible clinical use is considered. In this paper, we review the principal phage display strategies used to select anti-infectious mAbs, with particular attention focused on those used against hypervariable pathogens, such as HCV and influenza viruses.

Original languageEnglish
Pages (from-to)8273-8292
Number of pages20
JournalInternational Journal of Molecular Sciences
Volume13
Issue number7
DOIs
Publication statusPublished - 2012

Fingerprint

Bacteriophages
Monoclonal antibodies
pathogens
Cloning
Pathogens
antibodies
Organism Cloning
Display devices
Monoclonal Antibodies
viruses
optimization
Viruses
influenza
versatility
Respiratory Syncytial Viruses
Orthomyxoviridae
Technology
Palivizumab

Keywords

  • Biopanning
  • Hypervariable pathogens
  • Library construction
  • Monoclonal antibodies
  • Phage display

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Spectroscopy
  • Inorganic Chemistry
  • Catalysis
  • Molecular Biology
  • Computer Science Applications
  • Medicine(all)

Cite this

Phage display-based strategies for cloning and optimization of monoclonal antibodies directed against human pathogens. / Clementi, Nicola; Mancini, Nicasio; Solforosi, Laura; Castelli, Matteo; Clementi, Massimo; Burioni, Roberto.

In: International Journal of Molecular Sciences, Vol. 13, No. 7, 2012, p. 8273-8292.

Research output: Contribution to journalArticle

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