Pharmacokinetics of lopinavir determined with an ELISA test in youths with perinatally acquired HIV

Roberta Prinapori, Raffaella Rosso, Antonio Di Biagio, Franca Miletich, Elisa Furfaro, Lucia Taramasso, Francesca Ginocchio, Vania Giacomet, Loredana Nulvesu, Maria P ia Sormani, Irene Schiavetti, Alessio Signori, Laura De Hoffer, Claudio Viscoli

Research output: Contribution to journalArticle

Abstract

OBJECTIVE: To investigate the plasma levels of lopinavir by enzyme-linked immunosorbent assay (ELISA) in a cohort of patients who were vertically infected with human immunodeficiency virus 1 (HIV).

METHODS: Plasma levels of lopinavir (Cmin) were determined by ELISA test in patients treated with lopinavir/ritonavir-based combined antiretroviral therapy who had achieved virological response after 4 wk of therapy. Reference lopinavir concentrations were Cmin 1-8 μg/mL. Correlation between lopinavir plasma concentration and continuous variables was evaluated by mean of Pearson correlation coefficient. Differences in lopinavir (LPV) concentration for binary categorical variables were assessed by Mann-Whitney test, while for variables with more than two categories Kruskal-Wallis test was used.

RESULTS: Thirty-four patients were enrolled; median age was 133 mo (15-265). The median lopinavir dose tested was 383.5 mg/kg (IQR: 266.6-400 mg/kg), with a median plasma concentration of 8.8 μg/mL (IQR: 5-14 μg/mL). Lopinavir Cmin was  8 μg/mL. No significant correlations were found between plasma concentrations of lopinavir and the continuous variables considered in the study. A negative but, not completely significant, correlation was found between plasma drug concentration and body mass index (r = -0.29; p = 0.09).

CONCLUSIONS: The use of a simple and relatively cost-effective methodology might render therapeutic drug monitoring (TDM) appeal in the daily clinical practice.

Original languageEnglish
Pages (from-to)856-860
Number of pages5
JournalIndian Journal of Pediatrics
Volume81
Issue number9
DOIs
Publication statusPublished - Sep 1 2014

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Lopinavir
HIV-1
Pharmacokinetics
Enzyme-Linked Immunosorbent Assay
Ritonavir
Drug Monitoring
Body Mass Index
Costs and Cost Analysis

ASJC Scopus subject areas

  • Medicine(all)

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Pharmacokinetics of lopinavir determined with an ELISA test in youths with perinatally acquired HIV. / Prinapori, Roberta; Rosso, Raffaella; Di Biagio, Antonio; Miletich, Franca; Furfaro, Elisa; Taramasso, Lucia; Ginocchio, Francesca; Giacomet, Vania; Nulvesu, Loredana; Sormani, Maria P ia; Schiavetti, Irene; Signori, Alessio; De Hoffer, Laura; Viscoli, Claudio.

In: Indian Journal of Pediatrics, Vol. 81, No. 9, 01.09.2014, p. 856-860.

Research output: Contribution to journalArticle

Prinapori, R, Rosso, R, Di Biagio, A, Miletich, F, Furfaro, E, Taramasso, L, Ginocchio, F, Giacomet, V, Nulvesu, L, Sormani, MPI, Schiavetti, I, Signori, A, De Hoffer, L & Viscoli, C 2014, 'Pharmacokinetics of lopinavir determined with an ELISA test in youths with perinatally acquired HIV', Indian Journal of Pediatrics, vol. 81, no. 9, pp. 856-860. https://doi.org/10.1007/s12098-013-1198-1
Prinapori, Roberta ; Rosso, Raffaella ; Di Biagio, Antonio ; Miletich, Franca ; Furfaro, Elisa ; Taramasso, Lucia ; Ginocchio, Francesca ; Giacomet, Vania ; Nulvesu, Loredana ; Sormani, Maria P ia ; Schiavetti, Irene ; Signori, Alessio ; De Hoffer, Laura ; Viscoli, Claudio. / Pharmacokinetics of lopinavir determined with an ELISA test in youths with perinatally acquired HIV. In: Indian Journal of Pediatrics. 2014 ; Vol. 81, No. 9. pp. 856-860.
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abstract = "OBJECTIVE: To investigate the plasma levels of lopinavir by enzyme-linked immunosorbent assay (ELISA) in a cohort of patients who were vertically infected with human immunodeficiency virus 1 (HIV).METHODS: Plasma levels of lopinavir (Cmin) were determined by ELISA test in patients treated with lopinavir/ritonavir-based combined antiretroviral therapy who had achieved virological response after 4 wk of therapy. Reference lopinavir concentrations were Cmin 1-8 μg/mL. Correlation between lopinavir plasma concentration and continuous variables was evaluated by mean of Pearson correlation coefficient. Differences in lopinavir (LPV) concentration for binary categorical variables were assessed by Mann-Whitney test, while for variables with more than two categories Kruskal-Wallis test was used.RESULTS: Thirty-four patients were enrolled; median age was 133 mo (15-265). The median lopinavir dose tested was 383.5 mg/kg (IQR: 266.6-400 mg/kg), with a median plasma concentration of 8.8 μg/mL (IQR: 5-14 μg/mL). Lopinavir Cmin was  8 μg/mL. No significant correlations were found between plasma concentrations of lopinavir and the continuous variables considered in the study. A negative but, not completely significant, correlation was found between plasma drug concentration and body mass index (r = -0.29; p = 0.09).CONCLUSIONS: The use of a simple and relatively cost-effective methodology might render therapeutic drug monitoring (TDM) appeal in the daily clinical practice.",
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AU - Prinapori, Roberta

AU - Rosso, Raffaella

AU - Di Biagio, Antonio

AU - Miletich, Franca

AU - Furfaro, Elisa

AU - Taramasso, Lucia

AU - Ginocchio, Francesca

AU - Giacomet, Vania

AU - Nulvesu, Loredana

AU - Sormani, Maria P ia

AU - Schiavetti, Irene

AU - Signori, Alessio

AU - De Hoffer, Laura

AU - Viscoli, Claudio

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N2 - OBJECTIVE: To investigate the plasma levels of lopinavir by enzyme-linked immunosorbent assay (ELISA) in a cohort of patients who were vertically infected with human immunodeficiency virus 1 (HIV).METHODS: Plasma levels of lopinavir (Cmin) were determined by ELISA test in patients treated with lopinavir/ritonavir-based combined antiretroviral therapy who had achieved virological response after 4 wk of therapy. Reference lopinavir concentrations were Cmin 1-8 μg/mL. Correlation between lopinavir plasma concentration and continuous variables was evaluated by mean of Pearson correlation coefficient. Differences in lopinavir (LPV) concentration for binary categorical variables were assessed by Mann-Whitney test, while for variables with more than two categories Kruskal-Wallis test was used.RESULTS: Thirty-four patients were enrolled; median age was 133 mo (15-265). The median lopinavir dose tested was 383.5 mg/kg (IQR: 266.6-400 mg/kg), with a median plasma concentration of 8.8 μg/mL (IQR: 5-14 μg/mL). Lopinavir Cmin was  8 μg/mL. No significant correlations were found between plasma concentrations of lopinavir and the continuous variables considered in the study. A negative but, not completely significant, correlation was found between plasma drug concentration and body mass index (r = -0.29; p = 0.09).CONCLUSIONS: The use of a simple and relatively cost-effective methodology might render therapeutic drug monitoring (TDM) appeal in the daily clinical practice.

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