Pharmacological modulation of mitochondrial calcium uniporter controls lung inflammation in cystic fibrosis

Alessandro Rimessi, Chiara Pozzato, Lorenzo Carparelli, Alice Rossi, Serena Ranucci, Ida de Fino, Cristina Cigana, Anna Talarico, Mariusz R. Wieckowski, Carla M.P. Ribeiro, Claudio Trapella, Giacomo Rossi, Giulio Cabrini, Alessandra Bragonzi, Paolo Pinton

Research output: Contribution to journalArticlepeer-review

Abstract

Mitochondria physically associate with the endoplasmic reticulum to coordinate interorganelle calcium transfer and regulate fundamental cellular processes, including inflammation. Deregulated endoplasmic reticulum–mitochondria cross-talk can occur in cystic fibrosis, contributing to hyperinflammation and disease progression. We demonstrate that Pseudomonasaeruginosainfection increases endoplasmic reticulum–mitochondria associations in cystic fibrosis bronchial cells by stabilizing VAPB-PTPIP51 (vesicle-associated membrane protein–associated protein B–protein tyrosine phosphatase interacting protein 51) tethers, affecting autophagy. Impaired autophagy induced mitochondrial unfolding protein response and NLRP3 inflammasome activation, contributing to hyperinflammation. The mechanism by which VAPB-PTPIP51 tethers regulate autophagy in cystic fibrosis involves calcium transfer via mitochondrial calcium uniporter. Mitochondrial calcium uniporter inhibition rectified autophagy and alleviated the inflammatory response in vitro and in vivo, resulting in a valid therapeutic strategy for cystic fibrosis pulmonary disease.

Original languageEnglish
Article numbereaax9093
JournalScience advances
Volume6
Issue number19
DOIs
Publication statusPublished - May 2020

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Pharmacological modulation of mitochondrial calcium uniporter controls lung inflammation in cystic fibrosis'. Together they form a unique fingerprint.

Cite this