Pharmacological studies on isolated and perfused rat renal arteries

P. Hrdina, A. Bonaccorsi, S. Garattini

Research output: Contribution to journalArticlepeer-review

Abstract

A vascular smooth muscle preparation, the isolated perfused renal artery of rat, has been described. Noradrenaline, adrenaline, 5-HT, metaraminol and ATP contracted the isolated vessel in descending order of activity and showed a good dose-response relationship. Angiotensin and tyramine had very little effect. Dopamine, histamine, bradykinin, vasopressin, ADP, AMP, adenosine and 3′,5′-AMP did not show any contractile effect. Depolarization of the artery by K2SO4 abolished the effect of ATP and decreased the action of catecholamines, metaraminol and 5-HT. A low calcium medium made the artery insensitive to ATP, less sensitive to catecholamines and metaraminol but not to 5-HT. Phenoxybenzamine and phentolamine blocked the effect of sympathomimetics but not of ATP. 5-HT was inhibited by phenoxybenzamine but not by phentolamine. Cocaine and DMI potentiated the constrictor effect of noradrenaline, but not of adrenaline and 5-HT. DMI in higher concentrations (10-7 M, 10-6 M) showed an adrenolytic effect. This was, however, short-lived and, after withdrawal of the drug, was followed by a potentiating effect on noradrenaline. The NA potentiating effect of cocaine and DMI is probably due to their inhibitory action on the uptake of this amine by the arterial wall.

Original languageEnglish
Pages (from-to)99-108
Number of pages10
JournalEuropean Journal of Pharmacology
Volume1
Issue number2
Publication statusPublished - Mar 1967

Keywords

  • Adrenaline
  • ATP
  • Calcium
  • Desipramine
  • Metaraminol
  • Noradrenaline
  • Phenoxybenzamine
  • Phentolamine
  • Renal artery
  • Serotonin

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

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