Phase 1 clinical trial of live attenuated Shigella dysenteriae type-1 ΔicsA Δent Δfep ΔstxA: HgR oral vaccine SC599 in healthy human adult volunteers

Christine Sadorge, Anna Ndiaye, Nathalie Beveridge, Schale Frazer, Rafaela Giemza, Nathalie Jolly, Julie Johnson, Helen Liddy, Catherine A. Cosgrove, Paola Allavena, Alberto Mantovani, Stéphane Béchet, Annick Fontaine-Thompson, George E. Griffin, Francis Dupont, Philippe J. Sansonetti, David J M Lewis

Research output: Contribution to journalArticle

Abstract

Twenty-eight adults received between 102 and 108 colony forming units of live Shigella dysenteriae type-1 vaccine SC599, attenuated by deletion of invasion (icsA), iron chelation (ent, fep) and shiga toxin A-subunit (stxA) genes, followed by ciprofloxacin on day 4. Dose-independent diarrhea or change in bowel habit was seen in 3 subjects, without dysentery, vaccinaemia or serious adverse events. Hematology and biochemical parameters were unchanged. Doses of 105 or greater induced dose-independent SD1 lipopolysaccharide-specific antibody secreting cell (ASC) responses. Geometric mean number of IgA ASCs per 106 PBMCs for 105, 106, 107 and 108 groups were respectively 41, 8.8, 26 and 8.5. Serum antibody responses were seen in three subjects. SC599 appears immunogenic with maximum tolerated dose greater than 108 CFU.

Original languageEnglish
Pages (from-to)978-987
Number of pages10
JournalVaccine
Volume26
Issue number7
DOIs
Publication statusPublished - Feb 13 2008

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Keywords

  • Dysentery
  • Oral vaccine
  • Shigella dysenteriae

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Sadorge, C., Ndiaye, A., Beveridge, N., Frazer, S., Giemza, R., Jolly, N., Johnson, J., Liddy, H., Cosgrove, C. A., Allavena, P., Mantovani, A., Béchet, S., Fontaine-Thompson, A., Griffin, G. E., Dupont, F., Sansonetti, P. J., & Lewis, D. J. M. (2008). Phase 1 clinical trial of live attenuated Shigella dysenteriae type-1 ΔicsA Δent Δfep ΔstxA: HgR oral vaccine SC599 in healthy human adult volunteers. Vaccine, 26(7), 978-987. https://doi.org/10.1016/j.vaccine.2007.11.024